Fluorescent probes of the orientation of myosin regulatory light chains in relaxed, rigor, and contracting muscle.

The orientation of the light-chain region of myosin heads in relaxed, rigor, and isometrically contracting fibers from rabbit psoas muscle was studied by fluorescence polarization. Cysteine 108 of chicken gizzard myosin regulatory light chain (cgRLC) was covalently modified with iodoacetamidotetramethylrhodamine (iodo-ATR). Native RLC of single glycerinated muscle fibers was exchanged for labeled cgRLC in a low [Mg2+] rigor solution at 30 degrees C. Troponin and troponin C removed in this procedure were replaced. RLC exchange had little effect on active force production. X-ray diffraction showed normal structure in rigor after RLC exchange, but loss of axial and helical order in relaxation. In isolated myofibrils labeled cgRLC was confined to the regions of the sarcomere containing myosin heads. The ATR dipoles showed a preference for orientations perpendicular to the fiber axis, combined with limited nanosecond rotational motion, in all conditions studied. The perpendicular orientation preference was more marked in rigor than in either relaxation or active contraction. Stretching relaxed fibers to sarcomere length 4 microns to eliminate overlap between actin- and myosin-containing filaments had little effect on the orientation preference. There was no change in orientation preference when fibers were put into rigor at sarcomere length 4.0 microns. Qualitatively similar results were obtained with ATR-labeled rabbit skeletal RLC

Intertidal reef communities of the Marmion & Shoalwater Islands marine parks

Intertidal platform reefs are a distinctive feature of the Perth coastline. Occurring adjacent to shoreline beaches and also as isolated offshore reefs, these limestone platforms have been formed by wave action over many centuries. Rising and falling tides exert a major influence on the structure of intertidal reef communities, and this influence typically results in a distinctive distribution of organisms based on their tolerance to being exposed to the air when the tide is low. Those inhabiting the highest part of the rocky shore, and therefore exposed more often and for longer periods, are typically hardy, desiccation-resistant species. Those living further seaward are more frequently submerged, although even these organisms may be exposed to the drying sun during particularly low tides, or can be periodically buried by the deposition of shifting beach sand. During winter storms, large waves may crash onto these reefs, stripping away algae and dislodging animals. Yet despite such harsh conditions, intertidal reefs can support a diverse assemblage of algae and invertebrates. Intertidal reefs are recognised as key ecological values of the Marmion and Shoalwater Islands marine parks that are located adjacent to the Perth metropolitan area. Between 2009 and 2012, marine scientists from DEC’s Marine Science Program and the WA Herbarium worked with local marine park staff to survey some of the numerous intertidal reefs in these marine parks to improve our understanding of the communities they support

Fast track children's hearing pilot: final report of the evaluation of the pilot

This report presents key findings of the evaluation of the Fast Track children’s hearings pilot in Scotland1. The research was undertaken by staff at the Universities of Glasgow, Stirling and Strathclyde between February 2003 and January 2005

Algorithms for FFT Beamforming Radio Interferometers

Radio interferometers consisting of identical antennas arranged on a regular lattice permit fast Fourier transform beamforming, which reduces the correlation cost from $\mathcal{O}(n^2)$ in the number of antennas to $\mathcal{O}(n\log n)$. We develop a formalism for describing this process and apply this formalism to derive a number of algorithms with a range of observational applications. These include algorithms for forming arbitrarily pointed tied-array beams from the regularly spaced Fourier-transform formed beams, sculpting the beams to suppress sidelobes while only losing percent-level sensitivity, and optimally estimating the position of a detected source from its observed brightness in the set of beams. We also discuss the effect that correlations in the visibility-space noise, due to cross-talk and sky contributions, have on the optimality of Fourier transform beamforming, showing that it does not strictly preserve the sky information of the $n^2$ correlation, even for an idealized array. Our results have applications to a number of upcoming interferometers, in particular the Canadian Hydrogen Intensity Mapping Experiment--Fast Radio Burst (CHIME/FRB) project.Comment: 17 pages, 4 figures, accepted to Ap

Urinary Stone Proteins: An Update

The discovery of an organic component in kidney stones dates back to 1684. More than 150 years elapsed before the incrustation of this organic component, which is now called the matrix, was proposed as the mechanism of stone formation. The composition of the matrix remained largely unknown until the development of electron microscopy and the advances in biochemistry combined in the 1950\u27s to usher in the modern era of renal stone matrix investigation. Composed mainly of selectively incorporated proteins generally characterized by high glutamic and aspartic acid content and the frequent occurrence of y-carboxyglutamic acid, the matrix dis-plays a variable and complex composition and shares a few proteins in many stones. The embryonic stone may . first appear in the renal tubules where it can acquire the blood and cell membrane proteins recently found by analysis of stone protein extracts. The combination of supersaturation, an appropriate environment, the avail-ability of calcium binding proteins which may be abnormal, and the incorporation of proteins extracted from leukocytes and cell wall membranes may induce stone formation