The Bad Boy

Mr. Herbert Watts with his dog Antonio, called commonly Tony, walked into the park, Tony trotting leisurely at the end of his leash. He was a small dog of heterogeneous breed. One grandfather was known to be a full-blooded Irish terrier, but the rest of his grandparents were common dogs. Despite his questionable family tree, Antonio was a remarkable dog, according to the Watts\u27s. Mrs. Watts had just finished a new red sweater for Tony, and the air being chilly on this particular day, he was wearing it for the first time

Growth, survival, isolation and incidence of Y. enterocolitica in foods and other materials.

5 organisms/ml after48 h. Yersinia enterocolitica inoculated into boiled fish, egg, potato, rice, roast chicken and chocolate milk grew readily at all temperatures from 4 - 37°C. The organism survived without any change in count, for at least 8-9 weeks in these foods whilst stored at -20°C. Yersinia enterocolitica did not effect the pH of the food in which it was growing. The organism did not grow when inoculated into six mayonnaise sauces, with pH values of 2.9 - 4.8. Evaluations were made of agar and broth media and methods for the isolation of Y. enterocolitica from foods and other materials. Sixteen media commonly used for the isolation of other pathogens were compared with some eight media specially formulated for Y. enterocolitica. No medium was shown to be ideal. In an attempt to formulate or modify a new isolation medium the effects of dyes and other selective agents on the growth of Y. enterocolitica and other organisms was studied. Malachite green (0. 004%) incorporated into deoxycholate citrate sucrose agar was found to be inhibitory for many enterobacteria but not for Y. enterocolitica. This effect was shown to be related to the ratio of malachite green to sodium deoxycholate. Five studies involving 1004 foods and other materials were carried out to further evaluate various media and methods for the isolation of Y. enterocolitica as well as to investigate the incidence of the organism. The highest isolation rate was obtained from buffered peptone water incubated at 4°C and sub-cultured to lactose sucrose urea agar. Biochemical characterization and antibiotic sensitivity patterns were determined for all the presumptive Y. enterocolitica strains and the isolation of Y. enterocolitica sensu stricto was confirmed from 53 of 1004 (5. 3%) samples including 13/67 pasteurized milk, 7/119 beef, 16/159 pork, 6/40 sausages, 4/101 raw chicken, 4/14 sewer swabs, 2/140 animal feed and 1/1 cooked ham. Yersinia intermedia was isolated from seven samples and Y. frederiksenii from three samples. There was no significant difference in the antibiotic sensitivity patterns of the three species. Fourteen different serotypes were identified of which serotype 0:5 was the most commonly isolated. Other serotypes included 0:5,27, 0:6,30 and 0:8 each of which have been implicated in human illness. A few strains were rough and could not be serotyped and 17% of the isolates were not typable with the available antisera. Some foods contained multiple serotypes

How College Students’ Gender Stereotypes Affect Their Perceptions of Professors

A Research Methods Project supervised by Dr. Laura Wilson (Fall 2021)

SSR Variation in Important U.S. Maize Inbred Lines

Historically important public inbred lines continue to play an important role in maize (Zea maysL.) improvement in many different breeding programs. Their continued use means they have undergone numerous seed increases in diverse programs since their original release. Our objective was to estimate the level of genetic diversity among and within inbred lines from different sources using SSR markers. We sampled six inbred lines (B73, CM105, Mo17, Oh43, W153R, and Wf9) obtained from 14 sources (breeding programs). The data were analyzed by analysis of molecular variance (AMOVA), genetic diversity statistics, and genetic distance (Dice\u27s coefficient). Of the total variation observed in gene frequency, 87.8% was found among inbred lines, 7.6% among sources within inbred lines, and 4.6% within sources. Genotypes of identically named inbred lines from eight different sources differed slightly on the basis of 44 SSR loci. The mean genetic similarity between sources of the same inbred was greater than 85%. It can be concluded that although more diversity exists among these six inbred lines than within them, a small but significant amount of variation exists among seed sources within inbreds. This variation may have arisen through differences in seed maintenance, since we found no evidence to suggest high mutation rates or extensive outcrossing. The small but statistically significant level of variation raises concerns in germplasm conservation, mapping studies, marker development, and long-term recombinant inbred line development, especially when high resolution is desired

The Parenting Premmies Support Program: Designing and developing a mobile healthintervention for mothers of preterm infants

This article describes the development of the Parenting Premmies Support Program, a mobile health intervention designed to support mothers during the transitional time following their preterm infant’s discharge from hospital. Examples of how research teams give voice to the target population throughout the development and design of mHealth programs is largely missing from the literature. A detailed description of the steps taken in the development of the mHealth intervention that formed the support program is the intention of this paper. An exploratory, sequential, mixed-methods approach with a three-phase design was conducted. In each phase, the experience and perspectives of mothers of preterm infants were acknowledged and included. Phase one sought women’s accounts of their experience collected in semi-structured interviews (n = 9) and subject to a descriptive content analysis. In the second phase, a collaborative, stakeholder interrogation of issues was conducted to develop content of the mHealth protocol. In this phase, two interdependent procedures were used with two participant panels; a stakeholder panel (n = 10) undertook a series of face-to-face meetings, and a user group panel (n = 18) of women who had birthed a preterm infant up to 12 months before undertook an online Delphi survey. In phase three a pilot implementation of the program was undertaken with women whose preterm infants were being discharged home from hospital. The outcome was an mHealth protocol, a resource designed to support women by giving them information to understand and normalise their experience with their preterm infants, and to help them make decisions which may enhance responsive mothering. Collaborative research integrating user feedback in partnership with experts in the field increases the likelihood the final product will be of value and prove supportive and useful to the target audience

MossTalk Training for Word Retrieval: Generalization Across Semantic Categories

Little is known about the independent usefulness of MossTalk Words, a computerized training program for word retrieval, nor how training effects generalize to other untrained words. In a single-participant design, we investigated effects of independent training with MossTalk multi-mode matching exercises in four individuals with stroke-induced aphasia. Three of four participants improved in picture naming for trained words. One showed limited generalization to words in untrained semantic categories. Three improved in standardized testing, particularly for auditory comprehension, which is addressed in the matching exercises. Improvements are possible with independent training programs such as MossTalk Words

Developing generic online tutorials as a strategy for extending the use of WebCT

This paper is a progress report on the design and delivery of two short WebCT tutorials for students, as part of a wider strategy to encourage further use of online learning within the University. The tutorials address issues of concern to the Department and to the Higher Education community as a whole: the indiscriminate use of Internet resources by students, poor acknowledgement of sources and inaccurate citation practice. The various stages of design and delivery, as informed by guidance in action research methods are described. Issues surrounding the use of WebCT, including interactivity and accessibility, are discussed and some preliminary results of student evaluations and learning experiences are presented