1 research outputs found
Method for Real-Time Tissue Quantification of Indocyanine Green Revealing Optimal Conditions for Near Infrared Fluorescence Guided Surgery
Near infrared fluorescence guided
surgery (NIRFGS) offers better
distinction between cancerous and normal tissues compared to surgeries
relying on a surgeon’s senses of sight and touch. Because of
the greater accuracy in determining tumor tissue margins, NIRFGS within
clinics continues to grow. However, NIRFGS lacks standardization of
the indocyanine green (ICG) dose and the preoperative period allowed
after ICG administration. In an aim to find optimal doses and preoperative
periods for NIRFGS standardization, we developed a method that quantitatively
determines ICG levels within tissues in real-time. We find that not
only do the dose and the preoperative periods influence tumor-to-background
ratios (TBRs), but both also heavily influence subject-to-subject
variances of these ratios. Optimal detection conditions are observed
when larger than typical ICG doses are administered and longer than
typical preoperative periods are allowed. Larger doses lead to increased
TBRs, but longer preoperative periods are necessary to reduce TBR
variances to those observed when using smaller doses. Our results
suggest that a clinical investigation into maximum tolerable ICG doses
and prolonging preoperative periods in NIRFGS is warranted