New arylated benzo[h]quinolines induce anti-cancer activity by oxidative stress-mediated DNA damage

© 2016 The Author(s).The anti-cancer activity of the benzo[h]quinolines was evaluated on cultured human skin cancer (G361), lung cancer (H460), breast cancer (MCF7) and colon cancer (HCT116) cell lines. The inhibitory effect of these compounds on the cell growth was determined by the MTT assay. The compounds 3e, 3f, 3h and 3j showed potential cytotoxicity against these human cancer cell lines. Effect of active compounds on DNA oxidation and expression of apoptosis related gene was studied. We also developed a quantitative method to measure the activity of cyclin-dependent kinases-2 (CDK2) by western blotting in the presence of active compound. In addition, molecular docking revealed that benzo[h]quinolines can correctly dock into the hydrophobic pocket of the targets receptor protein aromatase and CDK2, while their bioavailability/drug-likeness was predicted to be acceptable but requires future optimization. These findings reveal that benzo[h]quinolines act as anti-cancer agents by inducing oxidative stress-mediated DNA damage

High-energy multiwatt femtosecond diode-pumped Yb:CaAlGdO4and Yb:CaF2regenerative amplifiers

We study and compare the performance of Yb:CaAlGdO4- and Yb:CaF2-based regenerative amplifiers at low (5 to 10 kHz) and high (up to 500 kHz) repetition rates. Both materials allow for pulse energies of <1 mJ with sub-400-fs at low repetition rates and up to 9.4 W of average output power at 500 kHz. Thanks to the good thermal properties of Yb:CaF2 and Yb:CALGO, the extracted energy has the potential to be significantly increased with further pump power scaling. Shorter pulses are also potentially achievable by optimizing the design of stretcher and compressor in order to better compensate higher-order dispersion and reduce nonlinear effects

High pulse energy multiwatt Yb:CaAlGdO4 and Yb:CaF2 regenerative amplifiers

We investigated and compared Yb:CaAlGdO4 and Yb:CaF2 regenerative amplifiers at repetition rates 5-10 kHz, a frequency range interesting for industrial applications requiring relatively high pulse energy. Both materials allow for pulse energies close to 1 mJ with sub-400-fs pulses. The two laser materials offer comparable performance in the pump power range investigated. The same regenerative amplifiers can be run up to 500 kHz for much faster material processing, with maximum output power of up to 9.4 W

28 W, 217-fs regenerative bulk amplifier based on Yb:CAlGdO4

Yb:CaAlGdO4 (Yb:CALGO) is a very promising material for high power ultrashort pulse generation, due to its broad emission bandwidth and good thermal properties. Here we report, to the best of our knowledge, the highest power and shorter pulses ever demonstrated from a Yb:CALGO-based regenerative amplifier. The system layout consists of a Yb:CALGO oscillator seeding a Yb:CALGO regenerative amplifier followed by a folded grating compressor. The Yb:CALGO oscillator provides approximately 650 mW output power in a 63 MHz repetition rate pulse train of 92-fs long pulses. The related spectrum is 12.5 nm wide (FWHM) and centered around 1050 nm. Average output powers as high as 36 W at 500 kHz are achieved out of the regenerative amplifier while pumping with 116 W at approximately 980 nm. A small roll-over in the regenerative output power is observed at maximum pump power. This is mostly due to a drift of the pump wavelength away from the maximum crystal absorption peak with increasing pump current. After compression, we obtained 28 W in a train of 217-fs long pulses, corresponding to a pulse energy higher than 50 µJ per pulse and a peak power above 0.25 GW. The pulse spectrum is centered at 1046 nm and is approximately 11 nm wide, corresponding to a time bandwidth product of 0.69. The beam quality factor stays below M2=1.15 up to the maximum output power level, confirming the outstanding thermal performances of the Yb:CALGO material. Experiments on further power up-scaling are in progress

Performance of the Yb:Lu2O3 laser crystal in diode-pumped femtosecond oscillators and high-power regenerative amplifiers

We investigated the performance of the laser gain medium Yb:Lu2O3 in a low-power oscillator and a high-power regenerative amplifier. Almost Fourier transform limited 78-fs pulses were demonstrated in the low-power, SESAM mode-locked oscillator pumped with a single-mode fiber-coupled laser diode. Exploiting the excellent thermomechanical properties of Yb:Lu2O3, we were able to achieve up to 22.2 W of average output power at a repetition rate of 500 kHz and 670-fs long pulses with the regenerative bulk amplifier. Experiments on further power up-scaling and pulse duration shortening are in progress. Up to 65 W were already proved in preliminary tests in cw regime