11 research outputs found

    Additional file 4: of A functional genomics screen identifies an Importin-α homolog as a regulator of stem cell function and tissue patterning during planarian regeneration

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    Lists all genes for which RNAi experiments were performed, with identifiers for the 5′- and 3′-end sequence reads or primer sequences for each clone used. The genes for which knockdown produced a phenotype are marked with the names they are referred to by in Table 1. (XLSX 41 kb

    Additional file 3: of A functional genomics screen identifies an Importin-α homolog as a regulator of stem cell function and tissue patterning during planarian regeneration

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    Provides identifiers for 5′ and 3′ EST sequence reads or primer sequences for clones used to make riboprobes. “Blastema”, “Neoblasts” and “CNS” columns indicate whether or not each gene was expressed in each of those tissues based on WISH. (XLSX 54 kb

    Additional file 2: of A functional genomics screen identifies an Importin-Îą homolog as a regulator of stem cell function and tissue patterning during planarian regeneration

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    Lists 1) all differentially expressed genes by gene ID with Blast2Go and KOG annotation for each gene; 2) normalized average expression levels for each gene tested in each sample type; 3) adjusted p -values for each comparison performed with differentially expressed genes highlighted in red. Where a significant change was observed, the “expression change” column provides a description of the direction of the change (up or down) and in which tissue type(s) (blastema or opposite side) the change was detected. (XLSX 1831 kb

    Additional file 5: of A functional genomics screen identifies an Importin-Îą homolog as a regulator of stem cell function and tissue patterning during planarian regeneration

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    Shows in situ hybridization to the genes (excluding ima-1) for which knockdown resulted in a phenotype. Animals were imaged from the ventral side and are shown with anterior to the left. Inset images for ddc and tph show photoreceptor expression imaged dorsally with anterior toward the top. (JPEG 583 kb

    Additional file 4: of Poly-GR dipeptide repeat polymers correlate with neurodegeneration and Clinicopathological subtypes in C9ORF72-related brain disease

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    Figure S3. Dot plot graph of semiquantitative assessment of neurodegeneration and DPR. Note that X axis is neurodegeneration score (0 to 3), Y-axis is density of DPR. FCtx - frontal cortex, DF - dentate fascia, CA4 - cornu ammonis sector 4. (TIF 1325 kb

    Additional file 7: of Poly-GR dipeptide repeat polymers correlate with neurodegeneration and Clinicopathological subtypes in C9ORF72-related brain disease

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    Figure S5 Comparison of immunostaining with aDMA between C9ORF72 cases and non- neurodegeneration control in parahippocampal cortex. The nuclear signal of aDMA is variable in both cases and controls. Note sparse cytoplasmic inclusions labeled with aDMA in in C9ORF72 cases (arrows). Scale bar represents 50 ΟM. (TIF 2898 kb

    Additional file 2: of Poly-GR dipeptide repeat polymers correlate with neurodegeneration and Clinicopathological subtypes in C9ORF72-related brain disease

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    Figure S2. Correlation between manual counts and positive pixel burden from color deconvolution in poly-GR staining. Plot shows the correlation of manual counts of neuronal cytoplasmic inclusions and positive pixel burden from color deconvolution in poly-GR staining. The line shows linear regression CD color deconvolution. Ctx frontal cortex, DF dentate fascia, CA - cornu ammonis, MCtx motor cortex. (TIF 2581 kb

    Additional file 5: of Poly-GR dipeptide repeat polymers correlate with neurodegeneration and Clinicopathological subtypes in C9ORF72-related brain disease

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    Figure S4. Western blot analysis of aDMA and sDMA in brains of C9FLTD-MND and sporadic FTLD-MND. The high molecular weight aDMA and sDMA signals are visible in c9FTD/ALS, but not in sporadic FTD/ALS cases. (TIF 2960 kb

    Additional file 3: of Poly-GR dipeptide repeat polymers correlate with neurodegeneration and Clinicopathological subtypes in C9ORF72-related brain disease

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    Table S1. Quantitative assessment of DPR density by color deconvolution algorithm in clinicopathologic subgroups of C9ORF72-related disease. In frontal cortex, p = 0.019, FTLD vs. FTLD-MND. In CA4, p = 0.055, FTLD vs. FTLD-MND. In CA2/3, p = 0.03, FTLD vs. FTLD-MND. Significant p-values (< 0.05) are indicated in bold. All variables were analyzed with Kruskal-Wallis ANOVA on Ranks and data are displayed as median (25th and 75th range). *Statistically significant p-value (p < 0.05); all p-value for ANOVA on Ranks comparison of all three groups. FCtx = frontal cortex, MCtx = Motor cortex, DF dentate fascia, CA hippocampal subfields. (DOCX 15 kb
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