Particle characterization.

<p>Particle characterization.</p

Thiolation reaction parameters^a.

<p>Thiolation reaction parameters<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0138761#t001fn001" target="_blank">^a</a>.</p

Antibody levels in mice vaccinated with un-conjugated or conjugated forms of Pfs25H, Pfs25M, and CSPM3, expressed as ELISA units (dilution sera to obtain an OD of 1).

<p>Mice were injected IM twice, 28 days apart, with 0.5 μg antigen content (* 2.5 μg for Pfs25H) of the vaccines adsorbed on 80 μg of Alhydrogel (** 45 μg of Alhydrogel for Pfs25M-EPA). Serum antibody levels were determined two weeks later by ELISA on plates coated with un-modified antigen. Dots represent ELISA units reported for individual mice. Center line represents the geometric mean, and error bars are 95% confidence levels. Statistics (Kruskal-Wallis with Dunn’s multiple comparisons): p < 0.05 for Pfs25H-EPA vs. Pfs25H; CSPM3-EPA vs. CSPM3- CSPM3; CSPM3-EPA vs. CSPM3.</p

Maleimide-modification reaction parameters^a.

<p>Maleimide-modification reaction parameters<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0138761#t002fn001" target="_blank">^a</a>.</p

Multiple thiol or maleimide linkers are non-specifically attached to lysine amines on the protein molecules by treatment with excess SATA or EMCS.

<p>Particles composed of A and B are formed by mixing thiolated protein with maleimide-modified protein.</p

Particle forming reaction parameters^a.

<p>Particle forming reaction parameters<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0138761#t003fn001" target="_blank">^<u>a</u></a>.</p

Inhibition of parasite growth <i>in vitro</i> with purified IgG from immunized rabbits.

<p>Y axis shows % inhibition, and X axis shows the concentration of AMA1-specific antibody used in the assay. Closed triangles represent individual rabbits vaccinated with un-conjugated AMA1. Open squares represents rabbits vaccinated with the AMA1-AMA1 particle.</p

Le Courrier

31 mars 18261826/03/31 (A0,N90)

Anti-Pfs25 or Pfs230D1 antibody titer of EPA conjugates of Pfs25 and Pfs230D1 synthesized by thioether (TE) and Amide (Am) conjugation chemistry.

<p>Mice were immunized with conjugates formulated in saline or Alhydrogel. Sera were analyzed on Day 42 after vaccination on Day 0 and 28. Dose: 0.5 μg/mouse.</p

Anti-Pfs25 antibody titer of EPA, TT or CRM conjugates of Pfs25 synthesized by thioether (TE) chemistry formulated in either Alhydrogel (AH) or AdjuPhos (AP).

<p>Immune sera were collected from Day 42 after two vaccinations were analyzed. Dose: 0.5 μg/mouse.</p