25 research outputs found
Other cell wall degrading proteins from MudPIT analysis.
<p>Other cell wall degrading proteins from MudPIT analysis.</p
Proteins associated with lignin metabolism from MudPIT analysis.
<p>Proteins associated with lignin metabolism from MudPIT analysis.</p
Most Abundant InterPro IDs Identified in MudPIT analysis.
<p>Most Abundant InterPro IDs Identified in MudPIT analysis.</p
Verification of lignocellulytic activity of <i>A. glabripennis</i> derived <i>F. solani</i> solid wood culture extracts through in vitro assays.
<p>Activities of cellulases, xylanases, and lignin peroxidases, Mn-dependent and independent peroxidases, as well as laccase were measured for <i>F. solani</i> extracts with each assay containing 50 µg of protein.</p>*<p>1 unit of activity = amount of enzyme that releases 1 µmol of reducing sugar per minute for reducing sugar assays and amount of enzyme that oxidizes 1 µmol substrate per min for peroxidase assays.</p
Heme staining and zymogram analysis of <i>A. glabripennis</i> derived <i>F. solani</i> solid wood culture extract.
<p>Twenty µg of fungal extract were loaded into each lane. Protein standard is on the left, with band sizes labeled (kDa). Lane A is a colloidal blue stained lane, and lane B is the corresponding heme stain/CMC/or xylan zymogram lane. For heme stain, a single band is present at approximately 70 kDa. For CMC zymogram six major zones of clearing are present at approximately 55, 32, 30, 27, 23 and 20 kDa and can be matched to protein bands on the colloidal blue stained lane. For birch wood xylan zymogram, many zones of clearing are present on the gel with major spots between 30 and 50 kDa. These correspond to a broad range of bands on the colloidal blue stained lane.</p
Glycoside hydrolase (GH) families detected in MudPIT analysis.
<p>Glycoside hydrolase (GH) families detected in MudPIT analysis.</p
Enrichment of GO Molecular Function terms in proteomic analysis.
<p>Bar graph represents the ratio of % composition of term in proteomic data vs. % composition in the genome annotation. Values over 1 (dotted line) are overrepresented in the proteomic data. Red line illustrates the relative abundance of the GO term in the proteomic data.</p
Proteinases and nitrogen recycling proteins identified from MudPIT data.
<p>Proteinases and nitrogen recycling proteins identified from MudPIT data.</p