3 research outputs found
ANTIBIOTIC SUSCEPTIBILITY PATTERNS OF ISOLATED BACTERIA FROM BILE FLUIDS OF PATIENTS WITH GALLSTONE DISEASE IN ISFAHAN CITY (IRAN)
Bacterial infections are one of the important agents in the creation of gallstones in the gallbladder. In recent years the spread of antibiotic-resistant bacteria such as extended-spectrum beta-lactamases (ESBL) is increasing and of concern in hospitalized patients worldwide. The purpose of this study was to investigate the antibiotic susceptibility patterns of isolated bacteria from the bile specimens of patients with chronic and acute cholecystitis who had been operated by single-incision laparoscopic cholecystectomy (SILC) in Isfahan (Iran) using an antibiogram susceptibility test and molecular technique. The bile fluids of 91 patients were obtained from the Al-Zahra hospital and were cultured on specific media for the isolation of Gram-negative and positive bacteria and the disk diffusion test was done to determine the antibiotic susceptibility patterns of isolated bacteria. Finally, bacterial DNA was extracted from the bile samples and polymerase chain reaction (PCR) was performed to investigate extended-spectrum beta-lactamases genes. The bacteria Escherichia coli, Klebsiella pneumoniae, Proteus spp. and Staphylococcus aureus were detected in bile specimens cultured with high frequency, and the results showed that biliary infection increased with aging in patients with gallstone disease operated by SILC. The results showed a high frequency of ESBL genes including TEM, SHV, and CTX-M in isolated bacteria (especially Escherichia coli and Klebsiella spp.). Thus, evaluating the antibiotic susceptibility patterns and screening of ESBLs bacteria in patients with gallstones are essential. Prescribing suitable drugs, designing good strategies, and informing the medical community could decrease bile infection and antibiotic-resistant bacteria in clinical centers and hospitals
Comparative Study of the Incidence of Port Site Infection in Disposable Ports and Reprocessed Disposable Ports in Laparoscopic Cholecystectomy
Introduction:Port site infection (PSI) is a rare complication of laparoscopic surgery that can reduce the benefits of this minimally invasive surgery and increase postoperative complications. As a variety of disposable ports (single-use ports) and reprocessed ports are used in laparoscopic surgeries, this study was conducted to compare the incidence of PSI in disposable ports and reprocessed disposable ports in laparoscopic cholecystectomy (LC). Materials and Methods:In this prospective cohort study, 473 patients were studied. Two hundred fifteen and two hundred fifty eight were in the disposable port group and reprocessed disposable port group, respectively. The demographic characteristics and incidence of superficial and deep infection were evaluated using the researcher-made checklist of infection assessment standards at intervals of 3 to 5 and 5 to 14 days following LC using call and physical examination on day 14. Data analysis was performed using descriptive statistics and statistical tests in SPSS software. Results:The incidence of PSI, 3 to 5 and 5 to 14 days after surgery in the reprocessed disposable port group was significantly higher than that in the disposable ports group. In both groups, all PSI accrued in the epigastric port area (where the gallbladder was removed) (P< 0.05). Conclusion:On the basis of the results, our study suggests using disposable ports instead, and in the case of using reprocessed disposable ports in LC, upgrading of disinfection and sterilization techniques is necessary
Antibiotic susceptibility patterns of isolated bacteria from bile fluids of patients with gallstone disease in Isfahan city (Iran)
Bacterial infections are one of the important agents in the creation of
gallstones in the gallbladder. In recent years the spread of
antibiotic-resistant bacteria such as extended-spectrum β-lactamases (ESBL)
is increasing and of concern in hospitalized patients worldwide. The purpose
of this study was to investigate the antibiotic susceptibility patterns of
isolated bacteria from the bile specimens of patients with chronic and acute
cholecystitis who had been operated by single-incision laparoscopic
cholecystectomy (SILC) in Isfahan (Iran) 2 using an antibiogram
susceptibility test and molecular technique. The bile fluids of 91 patients
were obtained from the Al-Zahra hospital and were cultured on specific media
for the isolation of Gram-negative and positive bacteria and the disk
diffusion test was done to determine the antibiotic susceptibility patterns
of isolated bacteria. Finally, bacterial DNA was extracted from the bile
samples and polymerase chain reaction (PCR) was performed to investigate
extended-spectrum β-lactamases genes. The bacteria Escherichia coli,
Klebsiella pneumoniae, Proteus spp. and Staphylococcus aureus were detected
in bile specimens cultured with high frequency, and the results showed that
biliary infection increased with aging in patients with gallstone disease
operated by SILC. The results showed a high frequency of ESBL genes including
TEM, SHV, and CTX-M in isolated bacteria (especially Escherichia coli and
Klebsiella spp.). Thus, evaluating the antibiotic susceptibility patterns and
screening of ESBLs bacteria in patients with gallstones are essential.
Prescribing suitable drugs, designing good strategies, and informing the
medical community could decrease bile infection and antibiotic-resistant
bacteria in clinical centers and hospitals