2 research outputs found
Antibacterial Activity of Geminized Amphiphilic Cationic Homopolymers
The
current study is aimed at investigating the effect of cationic
charge density and hydrophobicity on the antibacterial and hemolytic
activities. Two kinds of cationic surfmers, containing single or double
hydrophobic tails (octyl chains or benzyl groups), and the corresponding
homopolymers were synthesized. The antimicrobial activity of these
candidate antibacterials was studied by microbial growth inhibition
assays against <i>Escherichia coli</i>, and hemolysis activity
was carried out using human red blood cells. It was interestingly
found that the homopolymers were much more effective in antibacterial
property than their corresponding monomers. Furthermore, the geminized
homopolymers had significantly higher antibacterial activity than
that of their counterparts but with single amphiphilic side chains
in each repeated unit. Geminized homopolymers, with high positive
charge density and moderate hydrophobicity (such as benzyl groups),
combine both advantages of efficient antibacterial property and prominently
high selectivity. To further explain the antibacterial performance
of the novel polymer series, the molecular interaction mechanism is
proposed according to experimental data which shows that these specimens
are likely to kill microbes by disrupting bacterial membranes, leading
them unlikely to induce resistance
Adsorption and Orientation of 3,4-Dihydroxy‑l‑phenylalanine onto Tunable Monolayer Films
3,4-Dihydroxy-l-phenylalanine (l-DOPA) is considered
to be responsible for the mussel adhesion to a variety of surfaces.
A molecular level understanding of the interactions between DOPA molecules
and surfaces with different wettability and chemistry, however, posts
significant challenges to control marine antifouling. Here, different
self-assembled monolayers (SAMs) on gold surfaces were fabricated:
(i) OH-, (ii) COOH-, and (iii) CH<sub>3</sub>-terminations. The effect
of surface wettability and chemistry on the adsorption of DOPA upon
the series of surfaces was investigated in situ, showing that the
adsorbed mass was lower and the water content of DOPA layer was higher
on hydrophilic surfaces (including OH- and COOH-terminated SAMs) than
that on hydrophobic ones (including CH<sub>3</sub>-terminated SAMs
and gold surface). Direct evidence regarding the DOPA orientation
and the interaction between DOPA and film surfaces were obtained:
on the OH-terminated surface a flexible and loose structure formed
via coordinate hydrogen bonds of the hydroxyl end groups of the surface
interacting with carboxyl groups of DOPA, while for the CH<sub>3</sub>-terminated surface, DOPA molecules mainly adopt a flat conformation
due to the formation of hydrophobic “bonds” between
the hydrophobic functional groups of alkyl chains on surface and aromatic
rings of DOPA molecules. This study led a new insight into the adsorption
mechanisms based on the adsorption processes and layer structures,
and it proposed novel concepts for the design of antifouling and adhesive
surfaces