7 research outputs found
Diagnostic accuracy of the Enferplex Bovine TB antibody test using individual milk samples from cattle
Bovine tuberculosis is usually diagnosed using tuberculin skin tests or at post-mortem. Recently, we have developed a serological test for bovine tuberculosis in cattle which shows a high degree of accuracy using serum samples. Here, we have assessed the performance of the test using individual bovine milk samples. The diagnostic specificity estimate using the high sensitivity setting of the test was 99.7% (95% CI: 99.2-99.9). This estimate was not altered significantly by tuberculin boosting. The relative sensitivity estimates of the test using the high sensitivity setting in milk samples from comparative skin test positive animals was 90.8% (95% CI: 87.1-93.6) with boosting. In animals with lesions, the relative sensitivity was 96.0% (95% CI: 89.6-98.7). Analysis of paired serum and milk samples from skin test positive animals showed correlation coefficients ranging from 0.756-0.955 for individual antigens used in the test. Kappa analysis indicated almost perfect agreement between serum and milk results, while McNemar marginal homogeneity analysis showed no statistically significant differences between the two media. The positive and negative likelihood ratio were 347.8 (95% CI: 112.3-1077.5) and 0.092 (95% CI: 0.07-0.13) respectively for boosted samples from skin test positive animals. The results show that the test has high sensitivity and specificity in individual milk samples and thus milk samples could be used for the diagnosis of bovine tuberculosis.</p
Diagnostic accuracy of the Enferplex Bovine Tuberculosis antibody test in cattle sera
Bovine tuberculosis is a contagious bacterial disease of worldwide economic, zoonotic and welfare importance caused mainly by Mycobacterium bovis infection. Current regulatory diagnostic methods lack sensitivity and require improvement. We have developed a multiplex serological test for bovine tuberculosis and here we provide an estimate of the diagnostic accuracy of the test in cattle. Positive and negative reference serum samples were obtained from animals from Europe and the United States of America. The diagnostic specificity estimate was 98.4% and 99.7% using high sensitivity and high specificity settings of the test respectively. Tuberculin boosting did not affect the overall specificity estimate. The diagnostic sensitivity in samples from Mycobacterium bovis culture positive animals following tuberculin boosting was 93.9%.The relative sensitivity following boosting in tuberculin test positive, lesion positive animals and interferon gamma test positive, lesion positive animals was 97.2% and 96.9%Â respectively. In tuberculin test negative, lesion positive animals and in interferon gamma test negative, lesion positive animals, the relative sensitivity following tuberculin boosting was 88.2% and 83.6% respectively. The results show that the test has high diagnostic sensitivity and specificity and can detect infected animals that are missed by tuberculin and interferon gamma testing
Biotransformation of Monoterpenoids by Suspension Cultures of Lavandula angustifolia
Abstract Callus and suspension cultures of Lavandula angustifolia Mill. (Lamiaceae) were established and the effect of different culture media on growth rate was investigated. Terpenoids added to suspension culture to investigate their biotransformation. All samples were analyzed by gas chromatography (GC) and GC-mass spectroscopy (MS). Octan-1-ol, citronellol, linalool, borneol and geraniol were biotransformed products of octanal, citronellal, linalyl acetate, bornyl acetate and geranyl acetate, respectively. Citronellol, linalool, borneol, and menthol didn't change by L. angustifolia suspension cultures. Blue pigment production by cultures of L. angustifolia was also studied. Ester hydrolysis and oxidation were the main reactions which occurred in biotransformation process, which may be attributed to the presence of related or bifunctional enzymes. This technique is a possible way of the production of expensive or rare compounds from cheap and plentiful substrates
Biotransformation of Monoterpenoids by Suspension Cultures ofLavandula angustifolia: Monoterpenoid biotransformation
Callus and suspension cultures of Lavandula angustifoliaMill. (Lamiaceae)were established and the effect of different culture media on growth rate wasinvestigated. Terpenoids added to suspension culture to investigate their biotrans-formation. All samples were analyzed by gas chromatography (GC) and GC-massspectroscopy (MS). Octan-1-ol, citronellol, linalool, borneol and geraniol werebiotransformed products of octanal, citronellal, linalyl acetate, bornyl acetate andgeranyl acetate, respectively. Citronellol, linalool, borneol, and menthol didn'tchange by L. angustifoliasuspension cultures. Blue pigment production by culturesof L. angustifoliawas also studied. Ester hydrolysis and oxidation were the main reactions which occurred in biotransformation process, which may be attributed tothe presence of related or bifunctional enzymes. This technique is a possible wayof the production of expensive or rare compounds from cheap and plentiful substrates
Diagnostic accuracy of the Enferplex Bovine Tuberculosis antibody test in cattle sera
Abstract Bovine tuberculosis is a contagious bacterial disease of worldwide economic, zoonotic and welfare importance caused mainly by Mycobacterium bovis infection. Current regulatory diagnostic methods lack sensitivity and require improvement. We have developed a multiplex serological test for bovine tuberculosis and here we provide an estimate of the diagnostic accuracy of the test in cattle. Positive and negative reference serum samples were obtained from animals from Europe and the United States of America. The diagnostic specificity estimate was 98.4% and 99.7% using high sensitivity and high specificity settings of the test respectively. Tuberculin boosting did not affect the overall specificity estimate. The diagnostic sensitivity in samples from Mycobacterium bovis culture positive animals following tuberculin boosting was 93.9%.The relative sensitivity following boosting in tuberculin test positive, lesion positive animals and interferon gamma test positive, lesion positive animals was 97.2% and 96.9%Â respectively. In tuberculin test negative, lesion positive animals and in interferon gamma test negative, lesion positive animals, the relative sensitivity following tuberculin boosting was 88.2% and 83.6% respectively. The results show that the test has high diagnostic sensitivity and specificity and can detect infected animals that are missed by tuberculin and interferon gamma testing