RP-HPLC separation of detritylated 5mer Oxa-ODN (5′-GCOAT-3′) purified by Poly-Pak cartridge (), and DNA monomer, dCyd, dGuo, dThd, dOxo and dAdo formed by the digestion of with nuclease P1 and alkaline phosphatase ()

<p><b>Copyright information:</b></p><p>Taken from "Chemical synthesis and thermodynamic characterization of oxanine-containing oligodeoxynucleotides"</p><p>Nucleic Acids Research 2005;33(18):5771-5780.</p><p>Published online 11 Oct 2005</p><p>PMCID:PMC1255731.</p><p>© The Author 2005. Published by Oxford University Press. All rights reserved</p> HPLC condition: mobile phase; 100 mM TEAA solution (pH 7.0) with a gradient of CHCN [for (a), 7.6% (0 min)∼ 14% (40 min) (please see Materials and Methods) and for (b), 0% (0 min)∼20% (20 min)], with flow rate; 1 ml/min, column; Ultron VX-ODS columns [150 × 4.6 mm, 5 µm; Shinwa Co. (Kyoto, Japan)]

PAGE analysis of BER activities of AlkA, hMPG and HeLa CFE1s for Oxa and Oxa–Sp

<p><b>Copyright information:</b></p><p>Taken from "Repair activity of base and nucleotide excision repair enzymes for guanine lesions induced by nitrosative stress"</p><p>Nucleic Acids Research 2005;33(7):2181-2191.</p><p>Published online 14 Apr 2005</p><p>PMCID:PMC1079971.</p><p>© The Author 2005. Published by Oxford University Press. All rights reserved</p> () 25OXA, 25OXA–SP and 34MG (all 2 nM, and ) were incubated with the indicated amounts of AlkA and hMPG at 37°C for 30 min. After incubation, the reaction mixture was treated with 0.1 M NaOH to cleave AP sites, and products were separated by 16% denaturing PAGE. The nicked products due to β-elimination (upper bands) and β,δ-elimination (lower bands) are indicated by open brackets. () 25OXA and 25HX (both 2 nM, and ) were incubated in hMPG buffer with the indicated amounts of HeLa CFE1s at 37°C for 1 h. Products were analyzed as described above