1 research outputs found
Hybrid Nitrous Oxide Production from a Partial Nitrifying Bioreactor: Hydroxylamine Interactions with Nitrite
The
goal of this study was to elucidate the mechanisms of nitrous oxide
(N<sub>2</sub>O) production from a bioreactor for partial nitrification
(PN). Ammonia-oxidizing bacteria (AOB) enriched from a sequencing
batch reactor (SBR) were subjected to N<sub>2</sub>O production pathway
tests. The N<sub>2</sub>O pathway test was initiated by supplying
an inorganic medium to ensure an initial NH<sub>4</sub><sup>+</sup>-N concentration of 160 mg-N/L, followed by <sup>15</sup>NO<sub>2</sub><sup>–</sup> (20 mg-N/L) and dual <sup>15</sup>NH<sub>2</sub>OH (each 17 mg-N/L) spikings to quantify isotopologs of gaseous N<sub>2</sub>O (<sup>44</sup>N<sub>2</sub>O, <sup>45</sup>N<sub>2</sub>O, and <sup>46</sup>N<sub>2</sub>O). N<sub>2</sub>O production was
boosted by <sup>15</sup>NH<sub>2</sub>OH spiking, causing exponential
increases in mRNA transcription levels of AOB functional genes encoding
hydroxylamine oxidoreductase (<i>haoA</i>), nitrite reductase
(<i>nirK</i>), and nitric oxide reductase (<i>norB</i>) genes. Predominant production of <sup>45</sup>N<sub>2</sub>O among
N<sub>2</sub>O isotopologs (46% of total produced N<sub>2</sub>O)
indicated that coupling of <sup>15</sup>NH<sub>2</sub>OH with <sup>14</sup>NO<sub>2</sub><sup>–</sup> produced N<sub>2</sub>O
via <i>N</i>-nitrosation hybrid reaction as a predominant
pathway. Abiotic hybrid N<sub>2</sub>O production was also observed
in the absence of the AOB-enriched biomass, indicating multiple pathways
for N<sub>2</sub>O production in a PN bioreactor. The additional N<sub>2</sub>O pathway test, where <sup>15</sup>NH<sub>4</sub><sup>+</sup> was spiked into 400 mg-N/L of NO<sub>2</sub><sup>–</sup> concentration,
confirmed that the hybrid N<sub>2</sub>O production was a dominant
pathway, accounting for approximately 51% of the total N<sub>2</sub>O production