A hypomorphic vasopressin allele prevents anxiety-related behavior

In this study, microarray analysis, in situ hybridization, quantitative real-time PCR and immunohistochemistry revealed decreased expression of the vasopressin gene (Avp) in the hypothalamic paraventricular (PVN) and supraoptic (SON) nuclei of adult LAB mice compared to HAB, NAB (normal anxiety-related behavior) and HABxLAB F1 intercross controls, without detecting differences in receptor expression or density. By sequencing the regions 2.5 kbp up- and downstream of the Avp gene locus, we could identify several polymorphic loci, differing between the HAB and LAB lines. In the gene promoter, a deletion of twelve bp Δ(−2180–2191) is particularly likely to contribute to the reduced Avp expression detected in LAB animals under basal conditions. Indeed, allele-specific transcription analysis of F1 animals revealed a hypomorphic LAB-specific Avp allele with a reduced transcription rate by 75% compared to the HAB-specific allele, thus explaining line-specific Avp expression profiles and phenotypic features. Accordingly, intra-PVN Avp mRNA levels were found to correlate with anxiety-related and depression-like behaviors. In addition to this correlative evidence, a significant, though moderate, genotype/phenotype association was demonstrated in 258 male mice of a freely-segregating F2 panel, suggesting a causal contribution of the Avp promoter deletion to anxiety-related behavior

Advancing co-production for transformative change by synthesizing guidance from case studies on the sustainable management and governance of natural resources.

Co-production has become paramount for scientists, practitioners and social groups of Indigenous peoples and local communities of rural and urban areas to deliver transformative changes that enhance sustainability. Coproduction should result in knowledge that is credible, legitimate and usable to enable sustainable outcomes effectively. However, this is not always the case due to challenges related to differences between scientific and Indigenous and local knowledge, as well as inherent power imbalances. The literature emphasises that these challenges are often triggered by rigid scientific theories and postures, dominant practices, and time-money limitations that co-production projects involve. This happens despite the adoption of guidelines recommended in the literature. We investigate the role of these challenges and guidelines in the generation of credible, legitimate, usable, and effective knowledge. We analyse this role in 13 co-production cases focused on sustainable transformative changes linked with the management and governance of natural resources across the globe. Despite challenges varying between groups and contexts, credibility, usability, and effectiveness are promoted simultaneously, especially when co-production empowers social actors via legitimate processes. Scientists and practitioners do so, through creative and flexible reshaping of existing knowledge and worldviews with a focus on common goals that link sustainability and livelihoods. They conceptualise a mutual understanding of knowledge and that is deemed trustworthy feasible to use in their socioecological context. Our findings complement existing scholarship on co-production, exploring the credibility of situated knowledge and its practical effectiveness together with its commonly addressed legitimacy and usability. A focus on the practices of different actors, including dynamics that are external to co-production, and changes in the scientific and social status quo, are needed to advance co-production effectiveness

M.: An architecture for IP/LDP fastreroute using maximally redundant trees. Internet Draft

draft-ietf-rtgwg-mrt-frr-architecture-04 With increasing deployment of Loop-Free Alternates (LFA) [RFC5286], it is clear that a complete solution for IP and LDP Fast-Reroute is required. This specification provides that solution. IP/LDP Fast-Reroute with Maximally Redundant Trees (MRT-FRR) is a technology that gives link-protection and node-protection with 100 % coverage in any network topology that is still connected after the failure. MRT removes all need to engineer for coverage. MRT is also extremely computationally efficient. For any router in the network, the MRT computation is less than the LFA computation for a node with three or more neighbors. Status of This Memo This Internet-Draft is submitted in full conformance with the provisions of BCP 78 and BCP 79. Internet-Drafts are working documents of the Internet Engineering Task Force (IETF). Note that other groups may also distribute working documents as Internet-Drafts. The list of current Internet-Drafts is a

Can Volunteered Geographic Information be a participant in eEnvironment and SDI?

We investigate the potential role Volunteered Geographic Information (VGI) can play in eEvironment and various Spatial Data Infrastructures (SDI) on a local, regional, and national level. eEnvironment is the use and promotion of ICT for the purposes of environmental assessment and protection, spatial planning, and the sustainable use of natural resources. An SDI provides an institutionally sanctioned, automated means for posting, discovering, evaluating, and exchanging geospatial information by participating information producers and users. A key common theme shared by both definitions is public participation and user-centric services. We pose the research question: is VGI (an example of public participation and collaboration) is ready to participate in eEnvironment and SDI