10 research outputs found
Multifarious Polymorphism of a Multiblock Amphiphilic Macrocycle Bearing Thermally Responsive Polyether Segment
Formation of multiple crystalline
phases of a multiblock amphiphilic
macrocycle <b>AT2B</b> is demonstrated. <b>AT2B</b> forms
a single crystal (Cr-α) by vapor diffusion and shows reversible
single-crystal-to-single-crystal transition between two crystalline
phases (Cr-α and Cr-β) by a temperature change, and crystalline <b>AT2B</b> (Cr-β) melts at 422 K, and the cooling rate from
the melt influences the phase of the solid formed. By cooling at 1.0
K min<sup>–1</sup>, <b>AT2B</b> forms crystalline phases
(Cr-γ and Cr-δ), which are different from both Cr-α
and Cr-β. On the other hand, cooling at 2.0 K min<sup>–1</sup> results in the formation of an amorphous phase, and a mechanical
stress also triggers a crystal-to-amorphous solid transition. Interestingly,
the amorphous solid crystallizes to give the fifth crystalline phase
(Cr-γ) upon heating before melting. It is suggested that these
multiple phase transitions are driven by thermal conformational changes
at the tetraethylene glycol chains of <b>AT2B</b>
Contrasting Topological Effect of PEG-Containing Amphiphiles to Natural Lipids on Stability of Vesicles
Topology
of amphiphiles is important to control physicochemical
properties of supramolecular assemblies. Nature demonstrates higher
stability of membrane composed of lipids with a macrocyclic aliphatic
tail than those with linear tails, which likely results from the restricted
molecular structures of the macrocyclic lipids, allowing for closer
molecular packing. In contrast, here we report that a PEG-containing
macrocyclic amphiphile shows lower stability of vesicles than the
corresponding acyclic one. The macrocyclic amphiphile consists of
an aromatic hydrophobic part with chirality in which both ends are
strapped by octaethylene glycol via phosphoric ester groups, while
the acyclic amphiphile bears tetraethylene glycol chains attached
to both ends of the hydrophobic part. Because of the thermoresponsive
property of PEG to change its conformation, the hydrophobic part of
the macrocyclic amphiphile undergoes a larger thermal conformational
change than that of the acyclic one. In addition, the cyclic amphiphile
has a larger molecular area, which likely reduces the vesicular stability
compared with the acyclic one. Such a contrasting topological effect
caused by macrocyclization at the aliphatic part seen in the natural
system and at the hydrophilic part demonstrated in this study leads
to expand the molecular design of amphiphiles for both increasing
and decreasing the stability of vesicles by molecular topology
A plausible reaction mechanism of the intra- and intermolecular nucleophilic substitutions to prompt transetherification.
<p>A plausible reaction mechanism of the intra- and intermolecular nucleophilic substitutions to prompt transetherification.</p
Ether formation between tetraethylene glycol tosylate 2a and a) monoalcohol 8, b) propane-1,3-diol 11 and c) 2-(hydroxymethyl)propane-1,3-diol 13.
<p>Reaction time was 12 h. Yields were calculated based on the isolated amounts. ND: not detected.</p
MALDI-TOF-MS spectrum of the crude product extracted by CHCl<sub>3</sub> for the reaction in Table 1, Entry 1.
<p>Structures of 6 and 7 are shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0091912#pone-0091912-g003" target="_blank">Figure 3</a>. Matrix: α-cyano-4-hydroxycinnamic acid.</p
Mechano-Sensitive Synthetic Ion Channels
Mechanical
stress is a ubiquitous stimulus sensed by membrane proteins,
but rarely by synthetic molecules. Inspired by mechano-sensitive ion
channels found in cell membranes, tension-responsive transmembrane
multiblock amphiphiles were developed. In membranes, a single-transmembrane
amphiphile responds to both expanding and contracting tensions to
weaken and strengthen the stacking of membrane-spanning units, respectively,
and ion transportation is triggered by expanding tension to form a
supramolecular channel, while little transportation is observed under
a tensionless condition. In contrast, a three-transmembrane amphiphile
showed little spectroscopic response to tensions, likely due to weaker
stacking of membrane-spanning units than in the single-transmembrane
amphiphile. Nevertheless, the three-transmembrane amphiphile shows
ion transportation by forming a unimolecular channel even under a
tensionless condition, and the ion-transporting activity decreased
with expanding tension. Interestingly, the estimated operating force
of these synthetic systems was comparable to that of the mechano-sensitive
proteins. This study opens the door toward new mechano-sensitive molecular
devices
Ion Permeation by a Folded Multiblock Amphiphilic Oligomer Achieved by Hierarchical Construction of Self-Assembled Nanopores
A multiblock
amphiphilic molecule <b>1</b>, with a tetrameric
alternating sequence of hydrophilic and hydrophobic units, adopts
a folded structure in a liposomal membrane like a multipass transmembrane
protein, and is able to transport alkali metal cations through the
membrane. Hill’s analysis and conductance measurements, analyzed
by the Hille equation, revealed that the tetrameric assembly of <b>1</b> forms a 0.53 nm channel allowing for permeation of cations.
Since neither <b>3</b>, bearing flexible hydrophobic units and
forming no stacked structures in the membrane, nor <b>2</b>,
a monomeric version of <b>1</b>, is able to transport cations,
the folded conformation of <b>1</b> in the membrane is likely
essential for realizing its function. Thus, function and hierarchically
formed higher-order structures of <b>1</b>, is strongly correlated
with each other like proteins and other biological macromolecules
Reversible Ion Transportation Switch by a Ligand-Gated Synthetic Supramolecular Ion Channel
Inspired
by the regulation of cellular activities found in the
ion channel proteins, here we developed membrane-embedded synthetic
chiral receptors <b>1</b> and <b>2</b> with different
terminal structures, where receptor <b>1</b> has hydrophobic
triisopropylsilyl (TIPS) groups and receptor <b>2</b> has hydrophilic
hydroxy groups. The receptors have ligand-binding units that interact
with cationic amphiphiles such as 2-phenethylamine (PA). Conductance
study revealed that the receptors hardly show ion transportation at
the ligand-free state. After ligand binding involving a conformational
change, receptor <b>1</b> bearing TIPS termini displays a significant
current enhancement due to ion transportation. The current substantially
diminishes upon addition of β-cyclodextrin (βCD) that
scavenges the ligand from the receptor. Importantly, the receptor
again turns into the conductive state by the second addition of PA,
and the activation/deactivation of the ion transportation can be repeated.
In contrast, receptor <b>2</b> bearing the hydroxy terminal
groups hardly exhibits ion transportation, suggesting the importance
of terminal TIPS groups of <b>1</b> that likely anchor the receptor
in the membrane
Micrometer-Size Vesicle Formation Triggered by UV Light
Vesicle
formation is a fundamental kinetic process related to the
vesicle budding and endocytosis in a cell. In the vesicle formation
by artificial means, transformation of lamellar lipid aggregates into
spherical architectures is a key process and known to be prompted
by e.g. heat, infrared irradiation, and alternating electric field
induction. Here we report UV-light-driven formation of vesicles from
particles consisting of crumpled phospholipid multilayer membranes
involving a photoactive amphiphilic compound composed of 1,4-bisÂ(4-phenylethynyl)Âbenzene
(BPEB) units. The particles can readily be prepared from a mixture
of these components, which is casted on the glass surface followed
by addition of water under ultrasonic radiation. Interestingly, upon
irradiation with UV light, micrometer-size vesicles were generated
from the particles. Neither infrared light irradiation nor heating
prompted the vesicle formation. Taking advantage of the benefits of
light, we successfully demonstrated micrometer-scale spatiotemporal
control of single vesicle formation. It is also revealed that the
BPEB units in the amphiphile are essential for this phenomenon