The density of surviving hair cells in the utricles cultured with CoQ10 and neomycin.

<p>The density of surviving hair cells in the utricles cultured with CoQ10 and neomycin.</p

Protective effect of coenzyme Q10 against neomycin-induced cell death.

<p>Utricles were cultured for 24 h without neomycin (a, b), with neomycin (c, d) or with both neomycin and coenzyme Q10 (e–h). Death of striolar hair cells (red: calbindin) and extrastriolar hair cells (green: calmodulin) was inhibited by coenzyme Q10.</p

Inhibition of the production of 4-HNE in hair cells.

<p>Utricles were cultured for 12 h without neomycin (a, b), with neomycin (c, d), or with both neomycin and coenzyme Q10 (e, f). The hair cell layer was identified using Texas Red-conjugated phalloidin (red). 4-HNE was labeled using a specific antibody (green). Signals of 4-HNE were seen in utricle hair cells 12 h after exposure to neomycin. These 4-HNE signals were strongly inhibited by coenzyme Q10 treatment.</p

Fluorescence Intensity derived from 4-HNE.

<p>Fluorescence intensity of the immunohistochemistry was evaluated with the image analysis software: ImageJ. The fluorescence intensity derived from 4-HNE was significantly stronger in the utricles cultured with neomycin than without neomycin. The existance of coenzyme Q10 can inhibit the fluorescence intensity.</p

Additional file 3: of New paste for severe stomatitis in patients undergoing head-and-neck cancer radiotherapy and/or chemotherapy with oral appliance

Clinical trial results to date. (DOCX 19 kb