Additional file 10: Figure S1. of Mitochondrial genome sequences from wild and cultivated barley (Hordeum vulgare)

Alignment of protein sequences encoded in the mitochondrial genomes of grass species. (DOC 740 kb

Additional file 1: Figure S1. of Exome QTL-seq maps monogenic locus and QTLs in barley

Kernel color in the haploid mapping population. Figure S2. Depth of the mapped reads on the PRS in the exome-captured QTL-seq analysis for Blp mapping. Figure S3. Plots showing the SNP index of each chromosome generated by exome-captured QTL-seq analysis for Blp mapping in barley. Figure S4. Plots of the ΔSNP index of chromosome 1H generated by exome-captured QTL-seq analysis for Blp mapping. Figure S5. Infected leaf phenotypes. Figure S6. Depth of the mapped reads on PRS in the exome-captured QTL-seq analysis for net blotch resistance. Figure S7. Plots of the SNP index of each chromosome generated by exome-captured QTL-seq analysis for mapping of net blotch resistance. Figure S8. Plots of the ΔSNP index of chromosome 3H (left) and 6H (right) generated by exome-captured QTL-seq analysis for net blotch resistance. (DOCX 5291 kb

Additional file 2: Table S1. of Exome QTL-seq maps monogenic locus and QTLs in barley

Provisional exome sequences (PESs) based on Morex loci [3]. Table S2. RNA-seq data of Haruna Nijo used to restructure the pseudo reference sequence (PRS) in the QTL-seq analysis. Table S3. Number of aligned RNA-seq reads with PESs and detected SNPs against PES. Table S4. Number of reads and sequences used and mapped in the QTL-seq analysis of Blp. Table S5. Number of reads and sequences used and mapped in the QTL-seq analysis of net blotch resistance genes. (DOCX 32 kb

Synthesis and Thrombogenicity Evaluation of Poly(3-methoxypropionic acid vinyl ester): A Candidate for Blood-Compatible Polymers

A poly­(vinyl acetate) derivative, poly­(3-methoxypropionic acid vinyl ester) (PMePVE), was synthesized to develop a new candidate for blood compatible polymers. The monomer MePVE was synthesized by a simple two-step reaction, and then the MePVE was polymerized via free radical polymerization to obtain PMePVE. Human platelet adhesion tests were performed to evaluate the thrombogenicity, and the platelet adhesion was suppressed on the PMePVE-coated substrate. To determine the expression of the nonthrombogenicity of the PMePVE, the plasma protein adsorption and a conformationally altered state of fibrinogen were analyzed by a microBCA assay and enzyme-linked immunosorbent assay. The adsorption and denaturation of the plasma proteins were inhibited on the PMePVE; thus, PMePVE exhibited blood compatibility. A distinctive hydration water structure in the nonthrombogenic polymer, intermediate water (IW), was observed in the hydrated PMePVE by differential scanning calorimetry analysis. The nonthrombogenicity of PMePVE is considered to be brought about by the presence of IW

Additional file 5: Table S5. of Mitochondrial genome sequences from wild and cultivated barley (Hordeum vulgare)

Novel ORFs in the barley mitochondrial genome. (XLSX 15 kb

Additional file 2: Table S2. of Mitochondrial genome sequences from wild and cultivated barley (Hordeum vulgare)

Primer pairs used for SNP analysis. (XLSX 9 kb

Additional file 3: Table S3. of Mitochondrial genome sequences from wild and cultivated barley (Hordeum vulgare)

a Contigs of the mitochondrial genome of H602 generated by 454 sequencing, b Contigs of the mitochondrial genome of Haruna Nijo generated by 454 sequencing. (XLSX 13 kb

Additional file 7: Table S7. of Mitochondrial genome sequences from wild and cultivated barley (Hordeum vulgare)

Syntenic region between the mitochondrial genome and chloroplast genome in barley. (XLSX 12 kb

Maximum-likelihood phylogenetic tree of <i>Methylobacterium</i> isolates and related taxa, based on 16S rRNA gene sequences.

<p>Isolates from rice are colored in green and those from barley are in blue. Numbers in parentheses indicate isolates belonging to the same species, estimated by WC-MS analysis. For isolates from rice, the inoculation effect is not taken into account in the figure. <i>M aquaticum</i> strain 22A is taken as a representative strain for the <i>M</i>. <i>platani/aquaticum</i> cluster shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0129509#pone.0129509.g001" target="_blank">Fig 1</a>. Bootstrap percentages based on 1000 replicates are shown if greater than 80%. <i>Microvirga flocculans</i> TFB (AB098515) was used as an outgroup. Bar, 0.1 changes per nucleotide position.</p

Growth of <i>Methylobacterium</i>-inoculated barley.

<p>The data are presented as mean ± standard deviation, and analyzed with one-way ANOVA and Dunnett’s test. Statistical significance was indicated with</p><p>* (<i>p</i> < 0.05),</p><p>** (<i>p</i> < 0.01),</p><p>*** (<i>p</i> < 0.001),</p><p>**** (<i>p</i> < 0.0001).</p><p>Growth of <i>Methylobacterium</i>-inoculated barley.</p