Electric loading - Induced cracking behavior at electrode edges in PZT ceramics

Fatigue damage behavior under repeated electric loading was investigated on two kinds of PZT ceramics with discontinuous electrodes. Intergranular cracking was observed at the electrode edge in soft PZT under electric fields greater than ±400 V/mm. However, under the same loading conditions, no damage was observed in hard PZT. When cracking occurred, permittivity of specimens decreased with the number of cycles corresponding to the amount of mechanical damage. FEM analyses of the electroelastic field of the specimens showed that cracking due to cyclic electric loading was related to 180° domain switching caused by concentrated electroelastic field

Sirt1 expression is associated with CD31 expression in blood cells from patients with chronic obstructive pulmonary disease

Background: Cigarette smoke induced oxidative stress has been shown to reduce silent information regulator 1 (Sirt1) levels in lung tissue from smokers and patients with COPD patients. Sirt1 is known to inhibit endothelial senescence and may play a protective role in vascular cells. Endothelial progenitor cells (EPCs) are mobilized into circulation under various pathophysiological conditions, and are thought to play an important role in tissue repair in chronic obstructive lung disease (COPD). Therefore, Sirt1 and EPC-associated mRNAs were measured in blood samples from patients with COPD and from cultured CD34+ progenitor cells to examine whether these genes are associated with COPD development. Methods: This study included 358 patients with a smoking history of more than 10 pack-years. RNA was extracted from blood samples and from CD34+ progenitor cells treated with cigarette smoke extract (CSE), followed by assessment of CD31, CD34, Sirt1 mRNA, miR-34a, and miR-126-3p expression by real-time RT-PCR. Results: The expression of CD31, CD34, Sirt1 mRNAs, and miR-126-3p decreased and that of miR-34a increased in moderate COPD compared with that in control smokers. However, no significant differences in these genes were observed in blood cells from patients with severe COPD compared with those in control smokers. CSE significantly decreased Sirt1 and increased miR-34a expression in cultured progenitor cells. Conclusion: Sirt1 expression in blood cells from patients with COPD could be a biomarker for disease stability in patients with moderate COPD. MiR-34a may participate in apoptosis and/or senescence of EPCs in smokers. Decreased expression of CD31, CD34, and miR-126-3p potentially represents decreased numbers of EPCs in blood cell from patients with COPD

Glucosyl hesperidin exhibits more potent anxiolytic activity than hesperidin accompanied by the attenuation of noradrenaline induction in a zebrafish model

Anxiety is a symptom of various mental disorders, including depression. Severe anxiety can significantly affect the quality of life. Hesperidin (Hes), a flavonoid found in the peel of citrus fruits, reportedly has various functional properties, one of which is its ability to relieve acute and chronic stress. However, Hes is insoluble in water, resulting in a low absorption rate in the body and low bioavailability. Glucosyl hesperidin (GHes) is produced by adding one glucose molecule to hesperidin. Its water solubility is significantly higher than that of Hes, which is expected to improve its absorption into the body and enhance its effects. However, its efficacy in alleviating anxiety has not yet been investigated. Therefore, in this study, the anxiolytic effects of GHes were examined in a zebrafish model of anxiety. Long-term administration of diets supplemented with GHes did not cause any toxicity in the zebrafish. In the novel tank test, zebrafish in the control condition exhibited an anxious behavior called freezing, which was significantly suppressed in GHes-fed zebrafish. In the black-white preference test, which also induces visual stress, GHes-fed zebrafish showed significantly increased swimming time in the white side area. Furthermore, in tactile (low water-level stress) and olfactory-mediated stress (alarm substance administration test) tests, GHes suppressed anxious behavior, and these effects were stronger than those of Hes. Increased noradrenaline levels in the brain generally cause freezing; however, in zebrafish treated with GHes, the amount of noradrenaline after stress was lower than that in the control group. Activation of c-fos/ERK/Th, which is upstream of the noradrenaline synthesis pathway, was also suppressed, while activation of the CREB/BDNF system, which is vital for neuroprotective effects, was significantly increased. These results indicate that GHes has a more potent anxiolytic effect than Hes in vivo, which may have potential applications in drug discovery and functional food development

Influence of microstructure on fatigue property of ultra high-strength steels

Ultra-high-strength steels (with tensile strength higher than 980 MPa) are widely used in automobile manufacturing owing to their lightweight that contributes to fuel efficiency. The fatigue strength of ultra-high-strength steels with a notch tends to decrease, which is known as the effect of notch sensitivity. In this study, 4-point bending fatigue tests were performed to examine the fatigue strength and notch sensitivity of four steels; namely 590 MPa class steel, 980 MPa class martensitic steel, 980 MPa class bainitic steel, and 980 MPa class precipitation hardening steel plates with three different stress concentration factors. The results indicate that the fatigue strength and notch sensitivity of 980 MPa class steel specimens were higher than those of 590 MPa class steel specimens. The notch sensitivities of tested plate specimens were lower than those reported for cylindrical specimens of bainitic ultra-high-strength steels. Fatigue crack observation revealed that the cracks initiated in 590 MPa class steel, 980 MPa class bainitic, and martensitic steel propagated vertically from the lowest bottom of notch. Although similar initial crack propagation pattern was detected in precipitation hardening steel, the crack changed direction when it reached the central part of the specimen

Influence of microstructure on fatigue property of ultra high-strength steels

Ultra-high-strength steels (with tensile strength higher than 980 MPa) are widely used in automobile manufacturing owing to their lightweight that contributes to fuel efficiency. The fatigue strength of ultra-high-strength steels with a notch tends to decrease, which is known as the effect of notch sensitivity. In this study, 4-point bending fatigue tests were performed to examine the fatigue strength and notch sensitivity of four steels; namely 590 MPa class steel, 980 MPa class martensitic steel, 980 MPa class bainitic steel, and 980 MPa class precipitation hardening steel plates with three different stress concentration factors. The results indicate that the fatigue strength and notch sensitivity of 980 MPa class steel specimens were higher than those of 590 MPa class steel specimens. The notch sensitivities of tested plate specimens were lower than those reported for cylindrical specimens of bainitic ultra-high-strength steels. Fatigue crack observation revealed that the cracks initiated in 590 MPa class steel, 980 MPa class bainitic, and martensitic steel propagated southward from the lowest bottom of notch. Although similar initial crack propagation pattern was detected in precipitation hardening steel, the crack changed direction when it reached the central part of the specimen

Reciprocal regulation of TORC signaling and tRNA modifications by Elongator enforces nutrient-dependent cell fate

Nutrient availability has a profound impact on cell fate. Upon nitrogen starvation, wild-type fission yeast cells uncouple cell growth from cell division to generate small, round-shaped cells that are competent for sexual differentiation. The TORC1 (TOR complex 1) and TORC2 complexes exert opposite controls on cell growth and cell differentiation, but little is known about how their activity is coordinated. We show that transfer RNA (tRNA) modifications by Elongator are critical for this regulation by promoting the translation of both key components of TORC2 and repressors of TORC1. We further identified the TORC2 pathway as an activator of Elongator by down-regulating a Gsk3 (glycogen synthase kinase 3)-dependent inhibitory phosphorylation of Elongator. Therefore, a feedback control is operating between TOR complex (TORC) signaling and tRNA modification by Elongator to enforce the advancement of mitosis that precedes cell differentiation

The Rag GTPase-Ragulator complex attenuates TOR complex 1 signaling in fission yeast

Target of rapamycin complex 1 (TORC1) is an evolutionarily conserved protein kinase complex, whose activation in response to nutrients suppresses autophagy. In mammalian cells, amino-acid stimuli induce lysosomal translocation and activation of MTORC1 through the RRAG GTPase heterodimer, which is tethered to the surface of lysosomes by the Ragulator complex. Our recent study demonstrated that the fission yeast Schizosaccharomyces pombe also has a Ragulator complex that anchors the Gtr1-Gtr2 Rag GTPase heterodimer to the vacuole, a lysosome-like organelle. Unexpectedly, however, neither vacuolar localization nor activation of TORC1 is dependent on the Rag-Ragulator complex, which instead plays a critical role in attenuating TORC1 signaling. Our findings suggest dual functionality of the Rag GTPase in both activation and inactivation of TORC1