sj-xlsx-3-ajr-10.1177_19458924221132065 - Supplemental material for Circulating T Cell Subsets and ILC2s are Altered in Patients With Chronic Rhinosinusitis With Nasal Polyps After Dupilumab Treatment

Supplemental material, sj-xlsx-3-ajr-10.1177_19458924221132065 for Circulating T Cell Subsets and ILC2s are Altered in Patients With Chronic Rhinosinusitis With Nasal Polyps After Dupilumab Treatment by Toshiyuki Matsuyama, Hideyuki Takahashi, Hiroe Tada and Kazuaki Chikamatsu in American Journal of Rhinology & Allergy</p

sj-docx-5-ajr-10.1177_19458924221132065 - Supplemental material for Circulating T Cell Subsets and ILC2s are Altered in Patients With Chronic Rhinosinusitis With Nasal Polyps After Dupilumab Treatment

Supplemental material, sj-docx-5-ajr-10.1177_19458924221132065 for Circulating T Cell Subsets and ILC2s are Altered in Patients With Chronic Rhinosinusitis With Nasal Polyps After Dupilumab Treatment by Toshiyuki Matsuyama, Hideyuki Takahashi, Hiroe Tada and Kazuaki Chikamatsu in American Journal of Rhinology & Allergy</p

sj-jpg-2-ajr-10.1177_19458924221132065 - Supplemental material for Circulating T Cell Subsets and ILC2s are Altered in Patients With Chronic Rhinosinusitis With Nasal Polyps After Dupilumab Treatment

Supplemental material, sj-jpg-2-ajr-10.1177_19458924221132065 for Circulating T Cell Subsets and ILC2s are Altered in Patients With Chronic Rhinosinusitis With Nasal Polyps After Dupilumab Treatment by Toshiyuki Matsuyama, Hideyuki Takahashi, Hiroe Tada and Kazuaki Chikamatsu in American Journal of Rhinology & Allergy</p

sj-xlsx-4-ajr-10.1177_19458924221132065 - Supplemental material for Circulating T Cell Subsets and ILC2s are Altered in Patients With Chronic Rhinosinusitis With Nasal Polyps After Dupilumab Treatment

Supplemental material, sj-xlsx-4-ajr-10.1177_19458924221132065 for Circulating T Cell Subsets and ILC2s are Altered in Patients With Chronic Rhinosinusitis With Nasal Polyps After Dupilumab Treatment by Toshiyuki Matsuyama, Hideyuki Takahashi, Hiroe Tada and Kazuaki Chikamatsu in American Journal of Rhinology & Allergy</p

sj-jpg-1-ajr-10.1177_19458924221132065 - Supplemental material for Circulating T Cell Subsets and ILC2s are Altered in Patients With Chronic Rhinosinusitis With Nasal Polyps After Dupilumab Treatment

Supplemental material, sj-jpg-1-ajr-10.1177_19458924221132065 for Circulating T Cell Subsets and ILC2s are Altered in Patients With Chronic Rhinosinusitis With Nasal Polyps After Dupilumab Treatment by Toshiyuki Matsuyama, Hideyuki Takahashi, Hiroe Tada and Kazuaki Chikamatsu in American Journal of Rhinology & Allergy</p

Analysis of Helper T Cell Responses to Cry j 1-Derived Peptides in Patients with Nasal Allergy: Candidate for Peptide-Based Immunotherapy of Japanese Cedar Pollinosis

Background: Allergen specific immunotherapy is highly effective, but adverse events may occur during treatment. Peptide-based immunotherapy has been proposed as one of new strategies for reduction of allergic adverse reactions. We examined the possibility of candidate peptides for the development of peptide-based immunotherapy for Japanese cedar pollinosis. Methods: Twelve Cry j 1-specific T-cell lines were established from peripheral blood mononuclear cells (PBMC) of 12 patients with Japanese cedar pollinosis. Using these T-cell lines, 37 Cry j 1-derived overlapping peptides were assessed for their proliferative responses and cytokine production. Results: Four peptides corresponding to the Cry j 1 sequence were able to induce proliferative responses to more than one T-cell line: p61-80 (3/12; 25.0%); p115–132 (2/12; 16.6%); p206–225 (4/12; 33.3%); and p337–353 (5/12; 41.7%). Furthermore, T-cell lines generated from 11 of 12 donors (91.7%) responded to at least one of these four peptides. On the other hand, the pattern of cytokine production from Cry j 1-specific T-cell lines varied. Moreover, cytokine production patterns by stimulation with Cry j 1 peptide did not reflect those by stimulation with Cry j 1 protein. Conclusions: Our results suggest four Cry j 1-derived peptides (p61–80, p115–132, p206–225 and p337–353) may be considered to be the immunodominant T-cell epitopes of the Cry j 1 molecule, and can be useful for the design of peptide-based immunotherapy for the management of Japanese cedar pollinosis