Natural infection by Trypanosoma cruzi in triatomines and seropositivity for Chagas disease of dogs in rural areas of Rio Grande do Norte, Brazil

Submitted by Sandra Infurna ([email protected]) on 2018-09-18T15:13:39Z No. of bitstreams: 1 luisney_scoffier_etal_IOC_2018.pdf: 1445184 bytes, checksum: 05b433a68b0f8b8ec73da2709d11ad2e (MD5)Approved for entry into archive by Sandra Infurna ([email protected]) on 2018-09-18T15:24:52Z (GMT) No. of bitstreams: 1 luisney_scoffier_etal_IOC_2018.pdf: 1445184 bytes, checksum: 05b433a68b0f8b8ec73da2709d11ad2e (MD5)Made available in DSpace on 2018-09-18T15:24:52Z (GMT). No. of bitstreams: 1 luisney_scoffier_etal_IOC_2018.pdf: 1445184 bytes, checksum: 05b433a68b0f8b8ec73da2709d11ad2e (MD5) Previous issue date: 2018Universidade Federal Rural do Semi-Árido. Centro de Ciências Agrárias. Mossoró, RN, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil.Universidade Federal Rural do Semi-Árido. Centro de Ciências Agrárias. Mossoró, RN, Brasil.Universidade Federal Rural do Semi-Árido. Centro de Ciências Agrárias. Mossoró, RN, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Vigilância Entomológica em Diptera e Hemiptera. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Diptera. Rio de Janeiro, RJ, Brasil.Universidade Federal Rural do Semi-Árido. Centro de Ciências Agrárias. Mossoró, RN, Brasil.Universidade Federal Rural do Semi-Árido. Centro de Ciências Agrárias. Mossoró, RN, Brasil.Chagas disease is caused by the protozoa Trypanosoma cruzi. Its main reservoir is the domestic dog, especially in rural areas with favorable characteristics for vector establishment and proliferation. The aims of this study were to collect data, survey and map the fauna, and identify T. cruzi infection in triatomines, as well as to assess the presence of anti-T. cruzi antibodies in dogs in rural areas of the municipality of Mossoró, Brazil

Natural infection by Trypanosoma cruzi in triatomines and seropositivity for Chagas disease of dogs in rural areas of Rio Grande do Norte, Brazil

Abstract INTRODUCTION: Chagas disease is caused by the protozoa Trypanosoma cruzi. Its main reservoir is the domestic dog, especially in rural areas with favorable characteristics for vector establishment and proliferation. The aims of this study were to collect data, survey and map the fauna, and identify T. cruzi infection in triatomines, as well as to assess the presence of anti-T. cruzi antibodies in dogs in rural areas of the municipality of Mossoró, Brazil. METHODS: An active entomologic research was conducted to identify adult specimens through an external morphology dichotomous key. The analysis of natural infection by T. cruzi in the insects was performed by isolation in culture and polymerase chain reaction. The antibody testing for T. cruzi in dogs was performed by enzyme-linked immunosorbent assay and indirect immunofluorescence assay. RESULTS: A total of 68 triatomines were captured, predominantly the Triatoma brasiliensis brasiliensis (Neiva 1911) species. The vector mapping displayed areas with greater risk for parasite transmission. Of the examined triatomines (51 specimens), 41.2% (21/51) were positive on polymerase chain reaction, and all were negative on culture. In the serum testing, 11% (25/218) of dogs were seropositive, but no association was found between the serologic results and the presence and infection by T. cruzi in triatomines. CONCLUSIONS: This study demonstrated the movement of T. cruzi in the studied area, by the presence of vectors and naturally infected domestic reservoirs. The mapping of the studied rural area demonstrates the risk of disease transmission