A comparative study on the effect of ethanol extract of wild<em> Scrophularia deserti </em>and streptomycin on<em> Brucellla melitensis</em>

Introduction: Brucellosis or Malta fever is one of the most prevalent bacterial zoonosis which is transmitted to human being from animals. High fever, myalgia, and arthralgia of the large joints are the important symptoms. This study was aimed to evaluate the anti-brucellosis effect of ethanol extract of wild snapdragon on Brucella melitensis bacteria. Methods: This is an experimental in which after preparing the ethanol extract of wild Snapdragon (Scrophularia deserti), the anti-Brucellosis impressions of the extract on Brucella melitensis which is separated from goat&rsquo;s abortive fetus were studied by serial dilution and disc diffusion method. Results: In disc diffusion test, the disc containing 60 &mu;g/ml extract the inhibition zone was 9.7mm after 72, for streptomycin (20 &mu;g/disc) 13.3 mm and for streptomycin plus 60 &mu;g/disc extract discs it was 21.5 mm, after 72 hours incubation at 37 centigrade. After analyzing the data; the MIC for serial dilution test was 52 (576 &mu;g/ml) for the first repetition and 50 (576 &mu;g/ml) for the third repetition of the extract with OD600=1.56. For standard medicine "streptomycin" with OD600=1.67 it was 52 (360 &mu;g/ml). Conclusion: Ethanol extract of wild Scrophularia deserti is effective on Brucellla melitensis and its consumption might be useful in these patients.</p

Ethnobotanic study of medicinal plants in Urmia city: Identification and traditional using of antiparasites plants

Objective: To identify the native medicinal plants used in parasitic diseases treatment in Urmia. Methods: This study was conducted among 35 Urmia herbalists to identify medicinal plants used in parasitic diseases treatment. We used direct observation and interviews with collected herbarium specimens by native herbs commonly in the treatment of parasitic diseases. Questionnaires were included apothecary personal information and native plants list with information includes plant local name, plant parts used, method of their use and traditional therapies. Herbarium samples listed in the questionnaire collected from the area and were sent to agricultural research centers and Urmia University Faculty of Agriculture for genus and species determination. Results: Thirteen medicinal plants from six families for treatment of diabetes in Urmia were obtained from interviews. Most families have anti diabetic effect was included Asteraceae (36). The most used was boiling (65). Conclusions: In view of the findings of this study indicate that plants have the potential to be a parasitic infection so it is necessary ingredients of native plants be studied to demonstrate therapeutic effects and provide field work to evaluate the clinical effects of these herbs and ingredients they claim on parasitic diseases. © 2014 Asian Pacific Tropical Medicine Press

A comparative study on the effect of ethanol extract of wild Scrophularia deserti and streptomycin on Brucellla melitensis

Introduction: Brucellosis or Malta fever is one of the most prevalent bacterial zoonosis which is transmitted to human being from animals. High fever, myalgia, and arthralgia of the large joints are the important symptoms. This study was aimed to evaluate the anti-brucellosis effect of ethanol extract of wild snapdragon on Brucella melitensis bacteria. Methods: This is an experimental in which after preparing the ethanol extract of wild Snapdragon (Scrophularia deserti), the anti-Brucellosis impressions of the extract on Brucella melitensis which is separated from goat’s abortive fetus were studied by serial dilution and disc diffusion method. Results: In disc diffusion test, the disc containing 60 μg/ml extract the inhibition zone was 9.7mm after 72, for streptomycin (20 μg/disc) 13.3 mm and for streptomycin plus 60 μg/disc extract discs it was 21.5 mm, after 72 hours incubation at 37 centigrade. After analyzing the data; the MIC for serial dilution test was 52% (576 μg/ml) for the first repetition and 50% (576 μg/ml) for the third repetition of the extract with OD600=1.56. For standard medicine "streptomycin" with OD600=1.67 it was 52% (360 μg/ml). Conclusion: Ethanol extract of wild Scrophularia deserti is effective on Brucellla melitensis and its consumption might be useful in these patients