Species identification, antifungal susceptibility, and clinical feature association of Aspergillus section Nigri isolates from the lower respiratory tract

Species of Aspergillus section Nigri are generally identified by molecular genetics approaches, whereas in clinical practice, they are classified as A. niger by their morphological characteristics. This study aimed to investigate whether the species of Aspergillus section Nigri isolated from the respiratory tract vary depending on clinical diagnosis. Forty-four Aspergillus section Nigri isolates isolated from the lower respiratory tracts of 43 patients were collected from February 2012 to January 2017 at the National Hospital Organization (NHO) Tokyo National Hospital. Species identification was carried out based on β-tubulin gene analysis. Drug susceptibility tests were performed according to the Clinical and Laboratory Standards Institute (CLSI) M38 3rd edition, and the clinical characteristics were retrospectively reviewed. A. welwitschiae was isolated most frequently, followed by A. tubingensis. More than half of the A. tubingensis isolates exhibited low susceptibility to azoles in contrast to only one A. welwitschiae isolate. Approximately three quarters of the patients from whom A. welwitschiae was isolated were diagnosed with colonization, whereas more than half the patients from whom A. tubingensis was isolated were diagnosed with chronic pulmonary aspergillosis (CPA). More attention needs to be given to the drug choice for patients with CPA with Aspergillus section Nigri infection because A. tubingensis, which was found to be frequently azole-resistant, was the most prevalent in these patients

Dietary Lactoferrin Alleviates Age-Related Lacrimal Gland Dysfunction in Mice

BACKGROUND: Decrease in lacrimal gland secretory function is related to age-induced dry eye disease. Lactoferrin, the main glycoprotein component of tears, has multiple functions, including anti-inflammatory effects and the promotion of cell growth. We investigated how oral administration of lactoferrin affects age-related lacrimal dysfunction. METHODS AND FINDINGS: Twelve-month-old male C57BL/6Cr Slc mice were randomly divided into a control fed group and an oral lactoferrin treatment group. Tear function was measured at a 6-month time-point. After euthanasia, the lacrimal glands were subjected to histological examination with 8-hydroxy-2'-deoxyguanosine (8-OHdG) antibodies, and serum concentrations of 8-OHdG and hexanoyl-lysine adduct (HEL) were evaluated. Additionally, monocyte chemotactic protein-1(MCP-1) and tumor necrosis factor-α (TNF-α) gene expression levels were determined by real-time PCR. The volume of tear secretion was significantly larger in the treated group than in the control. Lactoferrin administration reduced inflammatory cell infiltration and the MCP-1 and TNF-α expression levels. Serum concentrations of 8-OHdG and HEL in the lactoferrin group were lower than those in the control group and were associated with attenuated 8-OHdG immunostaining of the lacrimal glands. CONCLUSION: Oral lactoferrin administration preserves lacrimal gland function in aged mice by attenuating oxidative damage and suppressing subsequent gland inflammation

Effectiveness of cyclic irrigation in reducing suspended solids load from a paddy-field district

The reduction of suspended solids, nutrients, and organic matter loads in drainage water from paddy fields is an important issue for water quality management in closed water areas in Japan. We evaluated the ability of cyclic irrigation to reduce the suspended solids load from paddy fields. In 2006 and 2007, we investigated water and mass balances during the irrigation period in a low-lying paddy-field district neighboring Lake Biwa, which is the largest lake in Japan. We confirmed that cyclic irrigation reduced effluent loads during the puddling season. With cyclic irrigation, 118 kg ha−1 of suspended solids was returned to the paddy fields in 2006 and 199 kg ha−1 in 2007. The effect of cyclic irrigation on the net suspended solids load can be represented by three ratios: the concentration ratio, which represents the ratio of the suspended solids concentration in drainage water to that in lake water; the cyclic irrigation ratio, which represents the ratio of the volume of reused water to that of irrigation water in cyclic irrigation; and the surplus irrigation water ratio, which represents the ratio of the volume of surplus irrigation water to that of irrigation water. The cyclic irrigation ratio and the surplus irrigation water ratio interact to determine the effect of cyclic irrigation on the net suspended solids load. Simultaneously increasing the cyclic irrigation ratio and decreasing the surplus irrigation water ratio will maximize the purification effect on drainage water from paddy fields

Relationship between increment of groundwater level at the beginning of irrigation period and paddy filed area in the Tedori River Alluvial Fan Area, Japan

There are many paddy fields and large amounts of groundwater in the Tedori River Alluvial Fan in Ishikawa Prefecture, Japan. Water infiltration from paddy fields during irrigation may significantly contribute to groundwater recharge. Groundwater recharge is known to be one outcome of paddy farming, and in general is usually related to land use. However, a decreased area of paddy fields because of socioeconomic factors such as urbanization and increasing area of fallow fields has possibly affected the groundwater environment. Evaluation of the quantitative effect of paddy fields on groundwater is necessary for groundwater conservation. This study examined the relationship between differences in the depth of groundwater from just before the irrigation period to just after the first irrigation of paddy fields (increments of groundwater levels) in observation wells and the area of paddy fields around each well. The paddy areas within circular buffer zones, which were delineated at 0.2 km intervals between 0.2 and 2.0 km centered on each observation well, were calculated. A positive relationship was found between the rise in groundwater and the area of paddy field within different buffer zones at most wells. In addition, in the middle or upper part of the fan, the effect of changes in the area of paddy fields surrounding the well on the groundwater level rise was greater than that on the lower part of the fan

Mutual influence between human activities and climate change in the Tibetan Plateau during recent years, Global and Planetary

Abstract It is well known that there is a vast expanse of grassland on the Tibetan Plateau, China. It is suggested that a major climatic factor limiting production of the grassland ecosystem is the low thermo-conditions on the Tibetan Plateau. An increase in temperature may therefore increase the productivity of grasslands on the Plateau. Monthly mean temperature and monthly precipitation data for recent years on the Tibetan Plateau were analyzed. It is clear that the increase in air temperature on the Plateau is greater than that in the whole China and East Asia. On the other hand, statistics show that the production of livestock or meat in the Tibetan Plateau has increased by one to three times since 1978, which means that there should be an equivalent increase in the consumption of plant biomass from the grassland. How is the ''increased biomass'' related to human activities and climate changes? This paper tries to clarify the relationship between human activities and climate change and to propose a possible answer by laying emphasis on the importance of grassland protection and CO 2 flux monitoring. It is suggested that there is a positive feedback in which degradation of grassland by overgrazing will increase potential evapotranspiration level thereby promoting the climate warming and the degradation process. This feedback should be considered seriously in the future.