Identification and Characterization of Skin Biomolecules for Drug Targeting and Monitoring by Vibrational Spectroscopy

The article discusses the application of vibrational spectroscopy techniques for in vivo identification and characterization of glucose biomolecules monitored in the skin of healthy, prediabetes and diabetes subjects; for molecular characterization of water and proteins in in vivo monitored patch tested inflamed skin of the patients with contact dermatitis; for description of nucleic acids and proteins at the molecular level with progression to malignancy in skin cancerous lesions. The results of the studies show new possibilities to assess activity levels of glucose metabolism in the skin tissue of healthy, prediabetes and diabetes subjects; activity and severity of inflammation; activity of the processes of carcinogenesis with regard to benign, premalignant and malignant transformation. Based on our findings, we suggest that vibrational spectroscopy might be a rapid screening tool with sufficient sensitivity and specificity to identify and characterize skin biomolecules in described diseases for drug targeting and monitoring by the pharmacological community

A compact synchroscan streak camera using a microchannel plate incorporated tube

A compact synchroscan streak camera, which incorporates a microchannel plates providing a high light gain, has been constructed. The camera has been operated in synchronism with a synchronous-passive hybrid mode-locked CW dye laser, and the overall time resolution has been 10.8 and 25.9 ps for a recording of ～160 and ～ lO^[8] cycles of dye laser pulses, respectively. In addition, by using the camera system with the dye laser a weak fluorescence profile (a quantum yield of ～10^[-3]) of an important biomolecule has been directly observed on a picosecond time scale

Picosecond time-resolved fluorescence spectroscopy of hematoporphyrin derivative

The picosecond time-resolved fluorescence decays I(t) and spectra I(λ, t) for hematoporphyrin derivative (HPD) in a phosphate buffer saline aqueous solution at different concentrations (8.4 x 10^[-6] ～ 8.4 x 10^[-3] M) are measured by a two-dimensional synchroscan streak camera with a mode-locked CW dye laser, and a new emission band (which we call the Y-band) is found at high concentration. It is shown that the fluorescence decays composed of fast and slow components at high concentration are due to the Y-band (120 ps lifetime) from head-to-tail aggregates including equilibrium dimer and stable dimer, and the usual band (3.6 ns lifetime) from monomer, respectively, and the latter band is dynamically quenched by the Förster type resonance energy transfer from the monomer to the aggregate. Furthermore, the measurement of static fluorescence spectra from human gastric cancers and the surrounding in vivo after HPD injection shows that a band corresponding to the Y-band from the aggregate appears at only the cancerous cells

Picosecond time-resolved fluorescence spectroscopy of hematoporphyrin derivative

The picosecond time-resolved fluorescence decays I(t) and spectra I(λ, t) for hematoporphyrin derivative (HPD) in a phosphate buffer saline aqueous solution at different concentrations (8.4 x 10^[-6] ～ 8.4 x 10^[-3] M) are measured by a two-dimensional synchroscan streak camera with a mode-locked CW dye laser, and a new emission band (which we call the Y-band) is found at high concentration. It is shown that the fluorescence decays composed of fast and slow components at high concentration are due to the Y-band (120 ps lifetime) from head-to-tail aggregates including equilibrium dimer and stable dimer, and the usual band (3.6 ns lifetime) from monomer, respectively, and the latter band is dynamically quenched by the Förster type resonance energy transfer from the monomer to the aggregate. Furthermore, the measurement of static fluorescence spectra from human gastric cancers and the surrounding in vivo after HPD injection shows that a band corresponding to the Y-band from the aggregate appears at only the cancerous cells