Immunohistochemical Localization of Amylase in Peri- and Tele-Insular Acinar Cells of the Human Exocrine Pancreas

An immunohistochemical localization of amylase was demonstrated in human pancreatic acinar cells using a commercial anti-human pancreatic amylase antibody. The immunofluorescence was mainly localized in the cell apices, and some differences in the intensity of the fluorescence was observed among the acinar cells in respect to their location from the islets of Langerhans. The peri-insular acinar cells showed a brighter fluorescence than the cells of tele-insular acini. This inhomogeneity of pancreatic amylase distribution in the human exocrine pancreas adds a further clue to the concept of insulo-acinar interaction.This study was supported by the Japanese Government Scholarship Program for Foreign Students from the Ministry of Education, Science and Culture, Japan

Light and Electron Microscopic Observation in a Case of Congenital Renal Proximal Tubular Dysfunction

We report a case of congenital renal proximal tubular dysfunction (CRPTD) accompanied by IgA nephropathy. The mesangial matrix was slightly increased with depositions of IgA and C3. Podocytes contained many clear vacuoles. Cuboidal cells, as well as squamous cells, lined the parietal epithelium of Bowman's capsule, although the functional or pathological significance of the cuboidal cells is unknown. The proximal tubular cells appeared to have a less-developed apical endocytic complex, basal infoldings and fewer lysosomes. These morphological changes may reflect proximal tubular dysfunction

細胞内小器官の直接3D観察

研究期間:平成10-12年度 ; 研究種目:基盤研究C2 ; 課題番号:10670017原著には既発表論文の別刷を含む

The Golgi apparatus of goblet cells in the mouse descending colon : three-dimensional visualization using a confocal laser scanning microscope

The three-dimensional structure of the Golgi apparatus was studied in goblet cells in lectin-stained sections of the mouse descending colon by using a confocal laser scanning microscope. In the lower part of the crypt, the Golgi apparatus formed a dome-or globe-like structure in the supranuclear region. The wall of the dome or the globe had some holes, one of which usually faced toward the nucleus and others toward the apical cytoplasm. Mucous granules seemed to be initially released into the interior of the dome and transported toward the apical cytoplasm through the holes. In the upper part of the crypt, on the other hand, the Golgi apparatus formed a cup- or funnel-like structure with a larger opening toward the cell apex and a smaller opening toward the nucleus. A large mass ofmucous granules occupied the inside of the cup to the apical cytoplasm. It is thought that the accumulation of mucous granules enlarges holes at the ceiling of the dome to form a large opening, which makes the configuration of the Golgi apparatus cup-shaped

Lymphocyte blastogenesis in normal and low birth weight infants and the effect of monocyte depletion on it

Peer Reviewe