Induction of T cell response.

<p>(A) <i>In vivo</i> immunization model with OVA-V antigen. (B, C) Proliferation of CD4+ T cells (B) and effector memory T cells (CD4+ TEM; C) activated with OVA-V-pulsed Dox-pDC. (D, E) Proliferation of CD4+CD8lo T cells (D) and effector memory T cells (CD4+CD8lo TEM; E) activated with OVA-V-pulsed Dox-pDC. (F-H) Frequency of Th1 (IFNγ+CD4+; F), Th17 (RORγt+CD4+; G) and CD8+ T cells (IFNγ+CD8+; H) polarized with OVA-LE-pulsed and TLR9-activated Dox-pDC or BM-pDC. Results are expressed as means ± SD from 3–9 mice per group. Statistical significance is indicated, ***(P<0.005) and ****(P<0.001).</p

Phenotype of immature Dox-pDC.

<p>(A) Scheme of generating Dox-pDC line. (B) Expression of pDC marker by the final 10 single cell clones. (C) IFNα secretion of the final 10 single cell clones. (D) Flow cytometric analysis of BM-pDC and Dox-pDC for different cell-specific markers. (E) Proliferation of Dox-pDC in the presence or absence of Dox and Flt3l and TLR9 activation detected with the viability proliferation dye 450 at day 0, 3, 5, 7 and analysed by flow cytometry. (F) Apoptosis staining of Dox-pDC in the presence or absence of Dox and Flt3l analysed by flow cytometry. (G) Doubling time of Dox-pDC calculated with an exponential growth equation. The figure shows representative results out of 2–4 experiments.</p