Longitudinal trend analysis of anti-SPSB3 level.

<p>Linear increment of ab-level after study inclusion in POAG + ODH patients with r-value ≥ 0.8 could be shown. Referring to time point 1 an increase of 22% in ab-level could be detected at the end point of the study at time point 4 (12 weeks after screening time point). In contrast, no relevant changes could be detected in the POAG group in the same time period.</p

Longitudinal Analysis of Serum Autoantibody-Reactivities in Patients with Primary Open Angle Glaucoma and Optic Disc Hemorrhage

<div><p>Background</p><p>The aim of our current investigation was to analyze the autoantibody-reactivities of primary open angle glaucoma patients with optic disc hemorrhage as possibly correlated to disease progression by means of a protein microarray approach.</p><p>Methods</p><p>Sera of patients with primary open angle glaucoma and optic disc hemorrhage (n = 16) were collected directly after study inclusion (0 weeks) and after 2 weeks, 4 weeks and 12 weeks. As a control group patients with primary open angle glaucoma (n = 18) were used (0 weeks and 12 weeks). Microarrays were incubated and occurring antibody-antigen-reactions were visualized with fluorescence labeled anti-human-IgG secondary antibodies. To detect changes in autoantibodies spot intensities were digitized and compared.</p><p>Results</p><p>With respect to the immunoreactivity at 0 weeks level increment of anti-adaptor protein 1 complex subunit mu-1 antibodies and anti-SPRY domain-containing SOCS box protein 3 antibodies in sera of primary open angle patients with optic disc hemorrhage was detected. Linear trend analysis revealed a positive correlation with r ≥ 0.8 between antibody-level and time course. Control group show no relevant changes in the same period. Significant changes were found in time point 4 comparison between patient groups in anti-adaptor protein 1 complex subunit mu-1-level (p = 0.01). No significant changes in visual acuity were found.</p><p>Conclusion</p><p>With this approach we were able to detect autoimmune reactivities in sera of patients with primary open angle glaucoma and optic disc hemorrhage compared to patients without optic disc hemorrhage. These antibodies could give further insights into the pathogenesis and the autoimmune component of glaucomatous optic neuropathy.</p></div

Influence of Corneal Opacity on Intraocular Pressure Assessment in Patients with Lysosomal Storage Diseases

<div><p>Aims</p><p>To investigate an influence of mucopolysaccharidosis (MPS)- and Morbus Fabry-associated corneal opacities on intraocular pressure (IOP) measurements and to evaluate the concordance of the different tonometry methods.</p><p>Methods</p><p>25 MPS patients with or without corneal clouding, 25 Fabry patients with cornea verticillata ≥ grade 2 and 25 healthy age matched controls were prospectively included into this study. Outcome measures: Goldmann applanation tonometry (GAT); palpatory assessment of IOP; Goldmann-correlated intraocular pressure (IOPg), corneal-compensated intraocular pressure (IOPcc), corneal resistance factor (CRF) and corneal hysteresis (CH) assessed by Ocular Response Analyzer (ORA); central corneal thickness (CCT) and density assessed with Pentacam. Statistical analysis was performed using linear mixed effect models and Spearman correlation coefficients. The concordance between tonometry methods was assessed using Bland-Altman analysis.</p><p>Results</p><p>There was no relevant difference between study groups regarding median GAT, IOPg, IOPcc and CCT measurements. The limits of agreement between GAT and IOPcc/IOPg/palpatory IOP in MPS were: [-11.7 to 12.1mmHg], [-8.6 to 15.5 mmHg] and [- 5.4 to 10.1 mmHg] respectively. Limits of agreement were less wide in healthy subjects and Fabry patients. Palpatory IOP was higher in MPS than in healthy controls and Fabry patients. Corneal opacity correlated more strongly with GAT, IOPg, CH, CRF, CCT and corneal density in MPS (r = 0.4, 0.5, 0.5, 0.7, 0.6, 0.6 respectively) than in Fabry patients (r = 0.3, 0.2, -0.03, 0.1, 0.3, -0.2 respectively). In contrast, IOPcc revealed less correlation with corneal opacity than GAT in MPS (r = 0.2 vs. 0.4).</p><p>Conclusions</p><p>ORA and GAT render less comparable IOP-values in patients suffering from MPS-associated corneal opacity in comparison to Fabry and healthy controls. The IOP seems to be overestimated in opaque MPS-affected corneas. GAT, IOPg and biomechanical parameters of the cornea correlate more strongly with the corneal clouding than IOPcc in MPS patients.</p><p>Trial Registration</p><p>ClinicalTrials.gov <a href="https://clinicaltrials.gov/ct2/show/NCT01695161" target="_blank">NCT01695161</a></p></div

Longitudinal trend analysis of anti-AP1M1 level.

<p>Linear increment of ab-level after study inclusion in POAG + ODH patients with r-value ≥ 0.8 could be shown. Referring to time point 1 an increase of 19% in ab-level could be detected at the end point of the study at time point 4 (12 weeks after screening time point). In contrast, no relevant changes could be detected in the POAG group in the same time period. Additionally, time point comparison between study groups revealed statistically significant higher level of anti-AP1M1-level in POAG patients with ODH at time point 4 (p = 0.01,Student’s t-test).</p

Age- and gender-distribution in patient cohort and sampling time points.

<p>A) The POAG + ODH group consists of 16 patients. Among these are 13 female persons and 3 male subjects with an overall mean age of 67.4 ± 9.7 years. For POAG group 11 female and 7 male patients were included. These 18 patients had an overall mean age at study inclusion of 67.4 ± 9.7 years. B) Blood collection and subsequent serum isolation was carried out at the beginning (Screening, 0 weeks) and at the end of the study (12 weeks) for both patient groups. POAG + ODH patients had additional sampling time points 14 days ± 3 days and 4weeks ± 7days after time point 1.</p

Bland-Altmann Plot for agreement between Goldmann applanation tonometry (GAT) and corneal-compensated IOP (IOPcc).

<p>Bland-Altmann Plot for agreement between Goldmann applanation tonometry (GAT) and corneal-compensated IOP (IOPcc).</p

Association of GAT, IOPcc, IOPg, CH, CRF, CCT and density with diagnostic group and the grade of the corneal clouding.

<p>Estimated means and 95% confidence intervals are shown.</p

Mean (estimated from linear mixed effects model) and corresponding 95% confidence interval of BCVA (decimal), spherical equivalent refraction (SEQ) and p-values.

<p>Mean (estimated from linear mixed effects model) and corresponding 95% confidence interval of BCVA (decimal), spherical equivalent refraction (SEQ) and p-values.</p

Goldmann applanation tonometry (GAT), corneal compensated IOP (IOPcc), Goldmann-correlated IOP (IOPg), corneal resistance factor (CRF), central corneal thickness (CCT) and corneal hysteresis (CH) in MPS type IV, VI and healthy controls in the right (OD) and the left (OS) eyes.

<p>Goldmann applanation tonometry (GAT), corneal compensated IOP (IOPcc), Goldmann-correlated IOP (IOPg), corneal resistance factor (CRF), central corneal thickness (CCT) and corneal hysteresis (CH) in MPS type IV, VI and healthy controls in the right (OD) and the left (OS) eyes.</p

Bland-Altmann Plot for agreement between Goldmann applanation tonometry (GAT) and Goldmann-correlated IOP (IOPg).

<p>Bland-Altmann Plot for agreement between Goldmann applanation tonometry (GAT) and Goldmann-correlated IOP (IOPg).</p