シンキ ファブネクチン ジュヨウタイ LAD-4 コウタイ ニヨル RL♂1 リンパシュ ノ カンゾウ テンイ ソガイ キジョ

Gazi MH and Ito M have previously shown that novel monoclonal antibody (mAb) LAD-4 inhibited the binding of RL♂1 lymhoma cells to fibronectin (FN) in vitro and partially those migration to the liver in vivo. In this study, I further examined the ability of LAD-4 to inhibit the RL♂1 liver metastasis and to have a cytopathic effect.　Histological examination revealed s.c. inoculation of BALB/c mice with RL♂1 followed i.p. injection of LAD-4 inhibited liver metastasis. Furthermore, flow cytometry (FCM) measurement of number of RL♂1 cells into liver confirmed LAD-4 obviously decreased them. LAD-4 improved survival of mice inoculated intravenously with RL♂1, but was not curative. LAD-4 decreased RL♂1 proliferation in vitro. Adsorbed LAD-4 on the micro-plates downregulated their proliferation under serum-free conditions, but FN did not. Treatment of RL♂1 with LAD-4 for 3h increased Annexin V-positive cells and caspase-3 activation. Moreover, FCM analysis using terminal deoxinucleotidyl transferase (TdT) revealed that 24h treatment of RL ♂1 with LAD-4 induced apoptotic DNA fragmentation. Inhibition of lymphoma cell adhesion to hepatocytic FN have been postulated the mechanism of LAD-4-mediated inhibition of RL♂1 liver metastasis. However, my results suggest that LAD-4 transduces an apoptotic signal through crosslinking of its receptor molecules and this may be another mechanism whereby LAD-4 inhibits RL♂1 liver metastasis.　Key words : fibronectin receptor, LAD-4, apoptosis, Annexin V, DNA fragmentatio

Evaluation of forest decontamination using radiometric measurements

An experiment has been conducted to evaluate the additional dose reduction by clear felling contaminated forestry in Fukushima Prefecture, Japan, and using the timber to cover the areas with wood chips. A portable gamma spectrometry system, comprising a backpack containing a 3x3” NaI(Tl) detector with digital spectrometer and GPS receiver, has been used to map dose rate and radionuclide activity concentrations before, after and at stages during this experiment. The data show the effect of the different stages of the experiment on dose rate at different locations around the site. The spectrometric data have allowed the assessment of the contributions of natural and anthropogenic radionuclides to the dose rate at different parts of the site before and after the experiment. This has clearly demonstrated the value of radiometric methods in evaluating remediation, and the effect of other environmental processes. The value of spectrometric methods which directly measure radionuclide concentrations has also been shown, especially through the identification of the contribution of natural and anthropogenic activity to the measured dose rate. The experiment has shown that clearing trees and applying wood chips can reduce dose rates by 10-15% beyond that achieved by just clearing the forest litter and natural redistribution of radiocaesium

Experimental characterization of the electronic structure of anatase TiO2: Thermopower modulation

Thermopower (S) for anatase TiO2 epitaxial films (n3D: 1E17-1E21 /cm3) and the gate voltage (Vg) dependence of S for thin film transistors (TFTs) based on TiO2 films were investigated to clarify the electronic density of states (DOS) around the conduction band bottom. The slope of the |S|-log n3D plots was -20 {\mu}V/K, which is an order magnitude smaller than that of semiconductors (-198 {\mu}V/K), and the |S| values for the TFTs increased with Vg in the low Vg region, suggesting that the extra tail states are hybridized with the original conduction band bottom.Comment: 11 pages, 4 figure

Photometric Studies of a WZ Sge-Type Dwarf Nova Candidate, ASAS160048-4846.2

We report on our time-resolved CCD photometry during the 2005 June superoutburst of a WZ Sge-type dwarf nova candidate, ASAS 160048-4846.2. The ordinary superhumps underwent a complex evolution during the superoutburst. The superhump amplitude experienced a regrowth, and had two peaks. The superhump period decreased when the superhump amplitude reached to the first maximum, successively gradually increased until the second maximum of the amplitude, and finally decreased again. Investigating other SU UMa-type dwarf novae which show an increase of the superhump period, we found the same trend of the superhump evolution in superoutbursts of them. We speculate that the superhump regrowth in the amplitude has a close relation to the increase of the superhump period, and all of SU UMa-type dwarf novae with a superhump regrowth follow the same evolution of the ordinary superhumps as that of ASAS 160048-4846.2.Comment: 7 pages, 4 figure

Phase Diagram for Self-assembly of Amphiphilic Molecule C12E6 by Dissipative Particle Dynamics Simulation

In a previous study, dissipative particle dynamics simulation was used to qualitatively clarify the phase diagram of the amphiphilic molecule hexaethylene glycol dodecyl ether (C12E6). In the present study, the hydrophilicity dependence of the phase structure was clarified qualitatively by varying the interaction potential between hydrophilic molecules and water molecules in a dissipative particle dynamics (DPD) simulation using the Jury model. By varying the coefficient of the interaction potential $x$ between hydrophilic beads and water molecules as x=-20, 0, 10, and 20, at a dimensionless temperature of T=0.5 and a concentration of amphiphilic molecules in water of phi=50% the phase structures grew to lamellar (x=-20), hexagonal (x=0), and micellar (x=10) phases. For x=20, phase separation occurs between hydrophilic beads and water molecules