HV Virginis and WZ Sge-Type Dwarf Novae

A dwarf nova HV Vir was observed photometrically for eight nights during the outburst in 1992 April - May. The star showed two distinct types of periodic variation: (1) 82.20-min (0.05708 d) double-humped variation with decaying amplitudes during the early stage of the outburst, and (2) 83.80-min (0.05820 d) superhumps in later stages. We attributed the former to "early superhumps", which are only seen in the earliest stage of WZ Sge-type outbursts. The superhump period and evolution of the superhumps, together with general characteristics of the light curve, make HV Vir a typical WZ Sge-type dwarf nova. HV Vir also showed a large increase of the superhump period during the superoutburst. Upon the recognition of the WZ Sge-type nature of an object previously considered as a nova, we present a comprehensive list of candidates for WZ Sge-type dwarf novae, and related systems.Comment: 24 pages, 10 figures, accepted for publication in Publ. Astron. Soc. Japa

Srs2 and RecQ homologs cooperate in mei-3-mediated homologous recombination repair of Neurospora crassa

Homologous recombination and post-replication repair facilitate restart of stalled or collapsed replication forks. The SRS2 gene of Saccharomyces cerevisiae encodes a 3′–5′ DNA helicase that functions both in homologous recombination repair and in post-replication repair. This study identifies and characterizes the SRS2 homolog in Neurospora crassa, which we call mus-50. A knockout mutant of N.crassa, mus-50, is sensitive to several DNA-damaging agents and genetic analyses indicate that it is epistatic with mei-3 (RAD51 homolog), mus-11 (RAD52 homolog), mus-48 (RAD55 homolog) and mus-49 (RAD57 homolog), suggesting a role for mus-50 in homologous recombination repair. However, epistasis evidence has presented that MUS50 does not participate in post-replication repair in N.crassa. Also, the N.crassa mus-25 (RAD54 homolog) mus-50 double mutant is viable, which is in contrast to the lethal phenotype of the equivalent rad54 srs2 mutant in S.cerevisiae. Tetrad analysis revealed that mus-50 in combination with mutations in two RecQ homologs, qde-3 and recQ2, is lethal, and this lethality is suppressed by mutation in mei-3, mus-11 or mus-25. Evidence is also presented for the two independent pathways for recovery from camptothecin-induced replication fork arrest: one pathway is dependent on QDE3 and MUS50 and the other pathway is dependent on MUS25 and RECQ2

Enhanced anti-HIV-1 activity of CC-chemokine LD78β, a non-allelic variant of MIP-1α/LD78α

AbstractWe compared the anti-HIV-1 activity of CC-chemokine LD78β with that of MIP-1α, another CC-chemokine which shows 94% sequence homology with LD78β. Despite its close similarity to MIP-1α, the anti-HIV-1 activity of LD78β appeared to be nearly 10 times higher than that of MIP-1α. Mutagenesis of MIP-1α showed that the N-terminal additional tetrapeptide, which was present in LD78β and absent in MIP-1α, is responsible for enhanced anti-HIV-1 activity. The N-terminal structure-function relationship of LD78β described here will be of value in understanding the chemokine-receptor interactions and designing anti-HIV-1 compounds based on LD78β

Low-density lipoprotein receptors play an important role in the inhibition of prostate cancer cell proliferation by statins

AbstractBackgroundThere are some reports about the antitumor effects of statins in these days. Statins decrease the level of cholesterol in the blood by inhibiting 3-hydroxy-3-methylglutaryl-coenzyme A reductase. Inhibition of this enzyme decreases intracellular cholesterol synthesis. Thus, the expression of low-density lipoprotein receptor (LDLr) is increased to import more cholesterol from the bloodstream. In this study, we assessed the effects of statins on the proliferation of prostate cancer cells, and studied the relationship between the expression of LDLr and the effects of statins.MethodsSimvastatin was used in the experiments. We studied the effect of simvastatin on PC-3 and LNCaP cell proliferation using the MTS assay, and evaluated the expression of LDLr after administration of simvastatin by quantitative polymerase chain reaction and Western blotting. Intracellular cholesterol levels in the prostate cancer cells were measured after administration of simvastatin. Furthermore, small interfering RNA (siRNA) was used to knockdown the gene expression of LDLr.ResultsIn PC-3 cells, simvastatin inhibited cell proliferation. In LNCaP cells, only a high concentration of simvastatin (100μM) inhibited cell proliferation. In LNCaP cells, the protein level of LDLr was increased by simvastatin. In PC-3 cells, the protein levels of LDLr were unregulated. In PC-3 cells, but not in LNCaP cells, intracellular cholesterol levels were significantly decreased by simvastatin. After knocking down LDLr expression by siRNA, intracellular cholesterol levels were decreased, and cell proliferation was inhibited by simvastatin in LNCaP cells.ConclusionSimvastatin inhibited prostate cancer cell growth by decreasing cellular cholesterol and could be more effective in androgen-independent prostate cancer, where there is loss of regulation of LDLr expression. LDLr was shown to play an important role in the statin-induced inhibition of prostate cancer cell proliferation. These results suggest that future studies evaluating the cholesterol-lowering effects of statin may lead to new approaches to the prevention and treatment of prostate cancer

Creation of Curved Surface by Lathe Turning -Development of CAM system using original tool layout-

AbstractThe machining of 3D curved surfaces with an un-axisymmetric axis by lathe turning is proposed considering the best machinable tool layout. The best offset tool layout from the central axis of a spindle enables us to machine curved surfaces and to obtain a long tool life for hard material workpieces using a rotary tool. A dedicated NC program for the 3D surface using the original CAM system has been developed and applied to what. The machining results and the validity of our system are evaluated in this paper

Isolation of an X-factor-dependent but porphyrin-positive Escherichia coli from urine of a patient with hemorrhagic cystitis

An Escherichia coli isolate was recovered from a 92-year-old female patient with urinary tract infection. Gram-stained preparation of the urine sediment manifested some gram-negative rod-shaped cells, and the urine specimen culture yielded nonhemolytic colonies on sheep blood agar plate. However, no visible colonies appeared on modified Drigalski agar plate. The isolate was finally identified as an X-factor-dependent E. coli. The interesting finding was that the isolate revealed a positive reaction for porphyrin test despite the requirement of hemin. This finding suggested that some pyrrol-ring-containing porphyrin compounds or fluorescent porphyrins had been produced as chemical intermediates in the synthetic pathway from delta-amino-levulinic acid (ALA), although the isolate should be devoid of synthesizing hems from ALA. This was the first clinical isolation of such a strain, indicating that the E. coli isolate should possess incomplete synthetic pathways of hems from ALA.ArticleJOURNAL OF INFECTION AND CHEMOTHERAPY. 19(4):764-766 (2013)journal articl