The NPL_all scores from initial non-parametric linkage analysis for the chromosomes showing suggestive linkage.

<p>Allele frequencies for the Affymetrix HMA10K Array were estimated using 20 affected individuals from six families and MERLIN was used for multipoint NPL analysis.</p

Non-parametric genome-wide linkage analysis results with MERLIN.

<p>Max NPL_all = maximum non-parametric linkage score when testing for allele sharing among affected individuals.</p

Finemapping of the 9q34 linkage peak region with microsatellite markers.

<p>The linkage area is marked by asterisks and the highest linkage peaks are highlighted in bold.</p>a<p>Genes in the mouse quantitative trait locus for susceptibility to group A streptococcal (GAS) infections on chromosome 2 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0056225#pone.0056225-Abdeltawab1" target="_blank">[18]</a>. (↑) Genes up regulated and, (↓) down regulated in GAS susceptible mouse strains.</p

The six most representative families used for initial linkage analysis.

<p>Arrows indicate probands and asterisks other family members studied.</p

Non-parametric linkage results from using additional microsatellite markers surrounding the suggested linkage peaks.

<p>The most significant locus is highlighted in bold. Physical coordinates were mapped against the GRCh37.2 human genome assembly. The deCODE genetic map was used for genetic locations <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0056225#pone.0056225-Kong1" target="_blank">[22]</a> and for markers absent on the deCODE map, genetic coordinates were estimated with linear interpolation using the markers’ physical coordinates. cM =  centiMorgan.</p><p>NPL_all = non-parametric linkage score when testing for allele sharing among affected individuals.</p

Affymetrix HMA250K results for 3q22.

<p>The haplotype that was significantly associated to erysipelas in Haploview is marked with bold letters in the “Associated allele” column. Significant p-values in Haploview or Haplotype pattern mining (HPM) for individual SNPs are also highlighted in bold. SNPs belonging to the associated haplotype and a significant p-value in Haploview, and with a significant p-value in HPM, and that showed shared heterozygosity among cases are marked with an asterisk.</p

SNP associations with preeclampsia (p≤10⁻⁶).

1<p>Physical coordinate based on NCBI reference assembly build 37.2.</p>2<p>Major allele/minor allele.</p>3<p>Odds ratio for the minor allele.</p

Quantile-quantile (Q-Q) plot of the observed GWAS p-values (−log₁₀P).

<p>Quantile-quantile (Q-Q) plot of the observed GWAS p-values (−log₁₀P).</p

Gene expression results of the <i>INHBB</i> structural locus ±500 kb.

1<p>Number of samples with a GenomeStudio ‘pDetection’ p-value≤0.05.</p>2<p>Computed transcript detection p-value.</p>3<p>False discovery rate detection p-value.</p>4<p>Mean average raw signal.</p>5<p>‘transcript∼preeclampsia’ regression p-value.</p>6<p>‘transcript∼preeclampsia+rs7579169’ regression p-value.</p>7<p>‘transcript∼preeclampsia+rs12711941’ regression p-value.</p>8<p>No regression analyses performed.</p

<i>INHBB</i> locus variants identified in a sample of the Australian cohort (n = 96).

<p>Identified variants were genotyped in Australian individuals passing GWAS quality control criteria (n = 1,078). Novel variants submitted to dbSNP are assigned with their ‘ss’ submission ID number.</p>*<p>Fisher's exact test p-value.</p>1<p>Major allele/Minor allele.</p>2<p>Hardy-Weinberg equilibrium p-value.</p>3<p>Preeclampsia dataset allele discordant to reference template allele.</p