Changes in daily sitting, standing, sit-to-stand transitions and stepping over time by condition.

<p>Changes in daily sitting, standing, sit-to-stand transitions and stepping over time by condition.</p

Additional file 1: of Cluster randomized controlled trial of a multilevel physical activity intervention for older adults

Table S1. Changes in exploratory outcomes overtime by intervention and control. (DOCX 16 kb

Flow diagram of progress through randomized pilot study.

<p>Flow diagram of progress through randomized pilot study.</p

Additional file 3: of Cluster randomized controlled trial of a multilevel physical activity intervention for older adults

Table S2. Adverse events by condition at 12 months. (DOCX 14 kb

Additional file 2: of Cluster randomized controlled trial of a multilevel physical activity intervention for older adults

Figure S1. Gender differences in physical activity between intervention and control conditions over time, adjusting for baseline demographic differences, nesting of days within people and people within sites. (DOCX 17 kb

Sedentary minutes accumulated in bouts of various lengths for each one hour period between 06:00 and 22:59.

<p>The number of sedentary minutes spent in bouts of (a) 1+, (b) 5+, (c) 10+, (d) 20+, (e) 30+, and (f) 60+ minutes are plotted across hours of the day for men and women.</p

Minutes in Sedentary Bouts^a, Per Day.

<p>^a Variables computed using daily-level data.</p><p>^b Means are adjusted for accelerometer wear time, physical functioning, depression, cognitive functioning, as well as age and gender where appropriate.</p><p>^c p-values were derived from F tests following linear mixed models that regressed sedentary time on measures of age, gender, physical functioning, cognitive functioning, depression and accelerometer wear time; Kenward-Roger approximations were used to estimate the denominator degrees of freedom.</p><p>Minutes in Sedentary Bouts<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0136161#t003fn001" target="_blank">^a</a>, Per Day.</p

Number of sedentary bouts started during each one hour period between 06:00 to 22:59.

<p>The number of (a) 1+, (b) 5+, (c) 10+, (d) 20+, (e) 30+, and (f) 60+ minute bouts are plotted across hours of the day for men and women.</p

Change in FMD during sitting conditions.

<p>Condition-associated change in FMD is represented by the ratio of FMD 2 (end of sitting period) to FMD 1 (baseline) and are shown by group (Panels A and B) and by individual (Panels C and D) using raw data (Panels A and C) and allometrically scaled data (Panels B and D) (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0188544#sec007" target="_blank">Materials and Methods</a> section). An FMD 2–to–FMD 1 ratio greater than 1 (dotted, horizontal line) indicates that the FMD response was greater at the completion of the sitting period relative to baseline. Box and whisker plots (Panels A and B): x = mean, line = median, dots above boxes are outliers. n = 10 for the control, 2-minute walking every hour, and 10-minute standing every hour conditions; n = 9 for the 2-minute standing every 20 minutes condition. <i>p</i>-value vs. the control condition. * Statistically significant after Bonferroni correction. Bonferroni-corrected cut-off for significance in 3-arm comparison with control was <i>p</i>< 0.0167.</p

BP and heart rate during sitting conditions.

<p>SBP (Panel A), DBP (Panel B), and heart rate (Panel C) measurements at each time point. Panel D. Average iAUC for DBP values across the entire 5-hr sitting period. <i>p</i>-value vs. control condition. Bonferroni-corrected cut-off for significance in 3-arm comparison with control was p< 0.0167. All data are means +/- SEM; n = 10 for the control, 2-minute walking every hour, and 10-minute standing every hour conditions; n = 9 for the 2-minute standing every 20 minutes condition.</p