Additional file 1: Figure S1. of Selective depletion of microglial progranulin in mice is not sufficient to cause neuronal ceroid lipofuscinosis or neuroinflammation

Progranulin mRNA, intracellular, and secreted protein levels correlate in primary microglia cultures. (A) Progranulin mRNA levels were reduced by approximately 50% in Het cultures compared to WT cultures and not detectable in GrnKO cultures. N = 2–3 wells/per genotype. (B) Progranulin protein quantified by ELISA on cell lysate and normalized to total protein per well shows a corresponding decrease of about 50% in Het cultures compared to WT cultures and negligible levels in GrnKO cultures. N = 6 wells/genotype. (C) Secreted progranulin detected by ELISA on conditioned media again shows progranulin reduced to approximately 50% in Het cultures compared to WT cultures and not detectable in GrnKO cultures. N = 6 wells/genotype. (PDF 90 kb