TLR3/7/8 activation in pregnant mice caused PE-like symptoms.

<p>Measures of (A) urinary protein concentration, (B) maternal body weight, (C) spleen weight/body weight, (D) litter weight, and (E) number of total pups/litter and fetal demise/litter in pregnant mice treated with the TLR3 agonist poly I:C, the TLR7 agonist R837, or the TLR7/8 agonist CLO97. Results are expressed as mean + SEM and n = 9 in each group. *p<0.05 vs. P.</p

Inflammation-related genes significantly (p<0.05 vs. P) altered in placentas of mice treated with a TLR3, TLR7, or TLR7/8 agonist during pregnancy.

<p>P = pregnant, P+TLR3 = pregnant+TLR3 agonist poly I:C, P+TLR7 = pregnant+TLR7 agonist R837, P+TLR7/8 = pregnant+TLR7/8 agonist CLO97. All p<0.05 vs. P, n>3 in each group.</p

Placental TLR3/7/8 activation in pregnant mice.

<p>Fold-increase and representative images and densitometric analyses of TLR3, TLR7, and TLR8 mRNA and protein levels, respectively, from 4 independent experiments in mice treated with (A) the TLR3 agonist poly I:C and (B) the TLR7 agonist R837 and the TLR7/8 agonist CLO97. Results are expressed as mean + SEM. *p<0.05 vs P.</p

TLR3/7/8 activation in human trophoblasts.

<p>Representative images and densitometric analyses of TLR3, TLR7, and TLR8 protein levels from 4 independent experiments for (A) the TLR3 agonist poly I:C, (B) the TLR7 agonist R837, and (C) the TLR7/8 agonist CLO97. Results are expressed as mean + SEM. *p<0.05 vs baseline.</p

TLR3/7/8 activation in mice caused pregnancy-dependent hypertension and endothelial dysfunction.

<p>Measures of (A) systolic blood pressure, (B) aortic endothelium-dependent relaxation induced by acetylcholine, and (C) aortic endothelium-independent relaxation induced by sodium nitroprusside in non-pregnant (top panels) and pregnant (bottom panels) mice treated with TLR3 agonist poly I:C, the TLR7 agonist R837, or the TLR7/8 agonist CLO97. Results are expressed as mean ± SEM and the n is given in parentheses. *p<0.05 vs P.</p

Increased TLR3/7/8 immunoreactivity and mRNA levels in placentas of women with preeclampsia.

<p>(A) Representative images where brown staining indicates immunoreactivity and blue indicates hematoxylin staining of nuclei in placentas from pregnant (P, n = 13, top panels) and preeclamptic (PE, n = 17, bottom panels) women. (B) mRNA levels of TLR3, TLR7, and TLR8 in placentas from PE and P women. Results are expressed as mean + SEM. *p<0.05 vs P.</p

Data for pregnant and preeclamptic patients.

<p>Data presented as mean ± SEM. SBP = systolic blood pressure, DBP = diastolic blood pressure, BMI = body mass index. *p<0.05 vs. pregnant.</p