Ig class switching to IgG1 is not reduced in Alkbh1^−/− splenic B cells.

<p>A. Flow cytometry results showing surface antibody expression four days after treatment to switch to IgG1. B. Analysis of flow cytometry data related to formation of IgG1 in ALKBH1 deficient splenic B cells normalized to the switching rate of Alkbh1^+/+ B cells. Four mice were used for each genotype and error bars represent standard deviations from these four independent experiments.</p

ALKBH1 deficient fibroblasts are not more sensitive to DNA modifying agents than hALKBH1 expressing cells.

<p>A. Expression of hALKBH1 in mouse Alkbh1^−/− fibroblasts. Lysates of indicated clones (20 µg) were analyzed by Western blot using a monoclonal anti-ALKBH1 antibody. The positions of two molecular mass markers are shown in kDa. B. Growth curves of Alkbh1^−/− fibroblasts and the same cells producing hALKBH1. Cells were plated at low cell density, harvested at the indicated time point by trypsinization and counted. Survival curves for Alkbh1^−/− fibroblasts expressing hALKBH1 or containing a vector control when treated with various concentrations of H₂O₂ (C) or MMS (D). Error bars indicate standard deviations derived from three (H₂O₂) or two (MMS) independent experiments.</p

Characterization of ALKBH1 deficient mice.

<p>A. Offspring distribution of crosses between heterozygous male and female mice. Numbers of pups from nine different litters are shown, demonstrating non-Mendelian inheritance of the knockout allele. B. Offspring distribution after crosses between Alkbh1^+/− females and Alkbh1^−/− males. C. Male-to-female ratios of Alkbh1^+/+, Alkbh1^+/−, and Alkbh1^−/− mice show a distorted sex ratio in favor of males for the knockout mice. D. Different sizes of Alkbh1^−/− and wild type (larger pup) littermates at two weeks of age. E. Weight development of Alkbh1^−/− and wild type pups. Alkbh1^−/− and wild type pups were derived from breeding either two ALKBH1 deficient or two wild type parent animals. For each time point, the weights of at least twelve animals were used to calculate the average and the error bars represent standard deviations.</p