Plant-derived BChE protects mice from SC-induced apnea.

<p>A Respiration rate of mice treated with SC followed by administration of pBChE or saline was monitored. Symbols represent individual animal subjects (<i>n</i> = 3 for each group). B The Kaplan–Meier estimates of survival reflecting survival of all pBChE-treated mice as opposed to 100% mortality among control, saline-treated subjects. Comparison by the Log-rank (Mantel-Cox) test was significant (<i>P</i><0.025).</p

Plant-derived BChE facilitate recovery of guinea pigs treated with sublethal dose of SC.

<p>Guinea pigs were injected with SC (0.167 mg/kg) followed by injection with BChE (<i>n</i> = 3, red) or saline (<i>n</i> = 3, blue) while monitoring oxygen saturation (A) and heart rate (B). Reported data points represent means±SEM. Means at each time point were compared by 2-way repeated ANOVA and P values are denoted on the graphs. Statistical analyses by t-test or 1-way ANOVA yielded very similar results. C Time to return to normal heart rate. Comparison by the Log-rank (Mantel-Cox) test was significant (<i>P</i><0.025).</p

Plant-derived BChE undergoes substrate activation by its succinylcholine substrate.

<p>Blue line represents data fitted by nonlinear regression to fit the equation: with the following parameters (±SEM): V_max = 2.45±0.16 µM/min, K_M = 57±7 µM, K_ss = 2.0±0.3 mM, and b = 2.9±0.1. <i>k</i>_cat = V_max/[BChE]_T was calculated to be 516±33 min⁻¹ based on the above V_max value and [BChE] = 4.74 nM.</p