Results of Programmed Evolution.

<p>(A) The starting population with equal amounts of all 24 strains was spread on LB agar plates with the indicated antibiotic and a disk treated as indicated. (B) Top row: spots of cells on LB agar with ampicillin for all 24 starting strains (left) and examples of clones after Programmed Evolution (right). Middle row: Agarose gels with PCR products to determine PCN for all 24 strains (left) and examples after Programmed Evolution (right). The 750 bp band for the low copy origin and the 500 bp band for the high copy origin are indicated by arrows. Bottom row: Agarose gels with PCR products to chaperone genotype for all 24 strains (left) and examples after Programmed Evolution (right). (C) The graph shows relative frequency of each of the genotype before (top) and after (bottom) Programmed Evolution. The order of chaperone plasmids along the left to right horizontal axis is pG-Tf2, pTf16, pG-KJE8, pGro7, pKJE7, and no chaperone. The order of genotype combinations along the other horizontal axis from back to front is high strength promoter/RBS + high copy origin; high strength promoter/RBS + low copy origin; low strength promoter/RBS + high copy origin; and low strength promoter/RBS + low copy origin.</p

Results of Programmed Evolution.

<p>The number and genotype of colonies analyzed after Programmed Evolution from three replicate plate experiments.</p

Origins of Replication Determine Plasmid Copy Number.

<p>The origins of replication used in the study are listed with their descriptions and part numbers in the Registry of Standard Biological Parts. The means and standard deviations of PCN values were determined by qPCR and yields of minipreps.</p

Starting Population for Programmed Evolution.

<p>(A) An ampicillin resistance plasmid carries variation in the strength of promoters and RBS elements as well as the low and high copy number origins of replication. (B) A chloramphenicol resistance plasmid carries chaperones DNA KJE, Trigger Factor, and Gro ESL chaperones individually and in two combinations (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0118322#sec004" target="_blank">Methods</a> for details).</p

Biosensor and Fitness Modules.

<p>(A) The Biosensor Module contains a promoter, a riboswitch that binds to theophylline, and a GFP gene. (B) Cells with the indicated genotypes were incubated with caffeine or theophylline. Fluorescence of cells grown in theophylline or caffeine was divided by absorbance at 590 nm (relative fluorescence) to correct for variation in cell density. (C) Relative fluorescence as a function of time in cells with and without the biosensor grown in 2.5 mM theophylline. (D) The Fitness Module contains a promoter, a riboswitch that binds theophylline, and the tetracycline resistance gene (<i>tetA</i>). (E) Cell growth in media containing tetracycline and either theophylline or caffeine as indicated.</p

Optimization of Metabolic Pathways.

<p>(A) Orthogonal metabolic output in a bacterial cell is depicted as a function (<i>f</i>) of the genetic circuit controlling metabolism and additional variables. (B) Two gene expression cassettes are drawn that encode enzymes controlling a metabolic pathway. Promoters, ribosome binding sites, and alleles for the two cassettes are chosen from a library of elements.</p