Primer extension activity of RT HIV-1 using the 17mer/44mer primer/template duplex

<p><b>Copyright information:</b></p><p>Taken from "Mechanism of the formation of DNA–protein cross-links by antitumor cisplatin"</p><p></p><p>Nucleic Acids Research 2007;35(6):1812-1821.</p><p>Published online 28 Feb 2007</p><p>PMCID:PMC1874601.</p><p>© 2007 The Author(s)</p> The experiments were conducted for the times indicated in the figure (5–90 min) using undamaged templates (lanes 2–5), the template containing single, site-specific 1,2-GG intrastrand CL of cisplatin (lanes 6–9) and the template containing single DPCL formed by the transformation of the template containing site-specific 1,2-GG intrastrand CL of cisplatin incubated with histone H1 (lanes 10–13). Lane 1, 17-mer primer. The pause sites opposite the platinated guanines and the nucleotide preceding the platinated guanines (thymine residue on the 3′ side of the CL) are marked 34, 33, 32, respectively. The nucleotide sequences of the templates and the primers are shown beneath the gels. See the text for other details

DPCL formation of unmodified and platinated oligodeoxyribonucleotide duplexes containing single, site-specific platinum adduct with KF assessed by SDS/PAA gel electrophoresis

<p><b>Copyright information:</b></p><p>Taken from "Mechanism of the formation of DNA–protein cross-links by antitumor cisplatin"</p><p></p><p>Nucleic Acids Research 2007;35(6):1812-1821.</p><p>Published online 28 Feb 2007</p><p>PMCID:PMC1874601.</p><p>© 2007 The Author(s)</p> Lanes: 1, 4, 7, 10, 13, the duplexes incubated with the protein for 4 h; 2, 5, 8, 11, 14 for 8 h; 3, 6, 9, 12, 15 for 24 h. Lanes: 1–3, the duplex TGT (20) containing monofunctional adduct of cisplatin; 4–6, the duplex TGT (20) containing monofunctional adduct of transplatin; 7–9, the duplex TGGT (20) containing 1,2-GG intrastrand CL of cisplatin; 10–12, the duplex TGTGT (20) containing 1,3-GTG intrastrand CL of cisplatin; 13–15, the duplex TGCT (20) containing interstrand CL of cisplatin. See the text for other details