Differences in candidate gene methylation between controls, T2D and MetS participants.

<p>Gene specific DNA methylation levels are shown as median (range). The Kruskal-Wallis Test was performed to test for differences between the three groups. The Wilcoxon Rank-Sum Test was performed to test for differences between two groups of participants.</p

DNA methylation levels of individual CpG loci located in the <i>KCNJ11</i>, <i>PPARγ</i>, <i>FTO</i> and <i>PDK4</i> genes.

<p>DNA methylation levels of specific CpG loci are shown as median (range). The Kruskal-Wallis Test was performed to test for differences between the three groups. The Wilcoxon Rank-Sum Test was performed to test for differences between two groups of participants. DNA methylation levels of individual CpG loci, located in the genes <i>KCNJ11</i>, <i>PPARγ</i>, <i>FTO</i> and <i>PDK4</i> were significantly different between groups of participants (p<b>≤</b>0.05). None of the other CpG loci included in this study were significantly different between our groups of participants (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0180955#pone.0180955.s003" target="_blank">S2 Table</a>).</p

Study participant characteristics.

<p>Characteristics of the participants in this study are shown as median (range). The participants were divided into three subgroups: control subjects, T2D patients and MetS patients. The Kruskal-Wallis Test was performed to test for differences between the three groups. The Wilcoxon Rank-Sum Test was performed to test for differences between two groups of participants.</p

Correlations between DNA methylation and characteristics of study participants.

<p>Gene specific DNA methylation levels are shown relative to patient characteristics.</p

Correction: Whole-Genome Saliva and Blood DNA Methylation Profiling in Individuals with a Respiratory Allergy

<p>Correction: Whole-Genome Saliva and Blood DNA Methylation Profiling in Individuals with a Respiratory Allergy</p

Comparison of methylation differences between RA cases and controls in PBMC and saliva as assessed by Illumina 450K bead chips and bisulfite pyrosequencing.

<p>Comparison of methylation differences between RA cases and controls in PBMC and saliva as assessed by Illumina 450K bead chips and bisulfite pyrosequencing.</p

The top 5 canonical pathways that show differential methylation between PBMC and saliva.

<p>The top 5 canonical pathways that show differential methylation between PBMC and saliva.</p

Identification of differentially methylated probes between RA cases and healthy controls (485 DMS in saliva and 437 DMS in PBMC).

<p>216 probes were in common in PBMC and saliva. The common probes showed the same polarity of methylation levels (<i>hypermethylated</i>, <b>contramethylated</b>, <u>hypomethylated</u>).</p

Network of genes involved in respiratory system development or inflammatory signaling.

<p>Twenty three of the focus genes are represented.</p

Differential methylation between blood cell types.

<p>Of the 216 DMS between allergy and control 55 sites are differently methylated between blood cell types (P<0.05).</p