Machine learning for emergent middleware

Highly dynamic and heterogeneous distributed systems are challenging today's middleware technologies. Existing middleware paradigms are unable to deliver on their most central promise, which is offering interoperability. In this paper, we argue for the need to dynamically synthesise distributed system infrastructures according to the current operating environment, thereby generating "Emergent Middleware'' to mediate interactions among heterogeneous networked systems that interact in an ad hoc way. The paper outlines the overall architecture of Enablers underlying Emergent Middleware, and in particular focuses on the key role of learning in supporting such a process, spanning statistical learning to infer the semantics of networked system functions and automata learning to extract the related behaviours of networked systems

Towards a target label-free suboptimum oligonucleotide displacement-based detection system

A novel method for the future development of label-free DNA sensors is proposed here. The approach is based on the displacement of a labelled suboptimum mutated oligonucleotide hybridised with the immobilised biotin-capture probe. The target fully complementary to the biotin-capture probe can displace the labelled oligonucleotide causing a subsequent decrease of the signal that verifies the presence of the target. The decrease of signal was demonstrated to be proportional to the target concentration. A study of the hybridisation of mutated and complementary labelled oligonucleotides with an immobilised biotin-capture probe was carried out. Different kinetic and thermodynamic behaviour was observed for heterogeneous hybridisation of biotin-capture probe with complementary or suboptimum oligonucleotides. The displacement method evaluated colourimetrically achieved the objective of decreasing the response time from 1 h for direct hybridisation of 19-mer oligonucleotides in the direct enzyme-linked oligonucleotide assay (ELONA) to 5 min in the case of displacement detection in the micromolar concentration range

Antibodies to Wheat High-Molecular-Weight Glutenin Subunits in Patients with Celiac Disease

10.1159/00033828