198 research outputs found

    Nonlinear elasticity of stiff biopolymers connected by flexible linkers

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    Networks of the biopolymer actin, cross-linked by the compliant protein filamin, form soft gels. They can, however, withstand large shear stresses due to their pronounced nonlinear elastic behavior. The nonlinear elasticity can be controlled by varying the number of cross-links per actin filament. We propose and test a model of rigid filaments decorated by multiple flexible linkers that is in quantitative agreement with experiment. This allows us to estimate loads on individual cross-links, which we find to be less than 10 pN. © 2009 The American Physical Society

    Microscale Flow Visualization of Nucleate Boiling in Small Channels: Mechanisms Influencing Heat Transfer

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    This paper describes the use of a new test apparatus employing flow visualization via ultra-high-speed video and microscope optics to study microscale nucleate boiling in a small, rectangular, heated channel. The results presented are for water. Because of confinement effects produced by the channel cross section being of the same nominal size as the individual vapor bubbles nucleating at discrete wall sites, flow regimes and heat transfer mechanisms that occur in small channels are shown to be considerably different than those in large channels. Flow visualization data are presented depicting discrete bubble/bubble and bubble/wall interactions for moderate and high heat flux. Quantitative data are also presented on nucleate bubble growth behavior for a single nucleation site in the form of growth rates, bubble sizes, and frequency of generation in the presence and absence of a thin wall liquid layer. Mechanistic boiling behavior and trends are observed which support the use of this type of research as a powerful means to gain fundamental insights into why, under some conditions, nucleate boiling heat transfer coefficients are considerably larger in small channels than in large channels

    Visualizing the strain field in semiflexible polymer networks: strain fluctuations and nonlinear rheology of F-actin gels

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    We image semi-flexible polymer networks under shear at the micrometer scale. By tracking embedded probe particles, we determine the local strain field, and directly measure its uniformity, or degree of affineness, on scales of 2-100 micron. The degree of nonaffine strain depends on polymer length and crosslink density, consistent with theoretical predictions. We also find a direct correspondence between the uniformity of the microscale strain and the nonlinear elasticity of the networks in the bulk.Comment: 9 pages (double-spaced) of text, 4 figures + 1 supplementary figur

    Glioma Expansion in Collagen I Matrices: Analyzing Collagen Concentration-Dependent Growth and Motility Patterns

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    Kaufman, L. J., C. P. Brangwynne, K. E. Kasza, E. Filippidi, V. D. Gordon, T. S. Deisboeck, and D. A. Weitz. “Glioma Expansion in Collagen I Matrices: Analyzing Collagen Concentration-Dependent Growth and Motility Patterns.” Biophysical Journal 89, no. 1 (July 2005): 635–50. doi:10.1529/biophysj.105.061994. -- C. P. Brangwynne, K. E. Kasza, and E. Filippidi, are with the Division of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts -- L. J. Kaufman, V. D. Gordon (currently with UT Austin), and D. A.Weitz are with the Department of Physics, Harvard University, Cambridge, Massachusetts -- T. S. Deisboeck is with the Molecular Neuro-Oncology Laboratory, Massachusetts General Hospital, Charlestown, Massachusetts and {Complex Biosystems Modeling Laboratory, Harvard-MIT (HST) Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Charlestown, Massachusetts -- L. J. Kaufman is with the Center for Imaging and Mesoscale Structures, Harvard University, Cambridge, Massachusetts; andWe study the growth and invasion of glioblastoma multiforme (GBM) in three-dimensional collagen I matrices of varying collagen concentration. Phase-contrast microscopy studies of the entire GBM system show that invasiveness at early times is limited by available collagen fibers. At early times, high collagen concentration correlates with more effective invasion. Conversely, high collagen concentration correlates with inhibition in the growth of the central portion of GBM, the multicellular tumor spheroid. Analysis of confocal reflectance images of the collagen matrices quantifies how the collagen matrices differ as a function of concentration. Studying invasion on the length scale of individual invading cells with a combination of confocal and coherent anti-Stokes Raman scattering microscopy reveals that the invasive GBM cells rely heavily on cell-matrix interactions during invasion and remodeling.Chemistr
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