Cadherin allele frequencies in F₂ progenies from four single-pair cross families.

1<p>Statistical significance was analyzed by Chi-square test with the predicted ratios of <i>cad^Ccad^C</i> : <i>cad^Ccad^G</i> : <i>cad^Gcad^G</i>  = 1∶2∶1 in the tested individuals of each treatment.</p

Relative levels of cadherin mRNA, normalized to the ß-actin mRNA, in the midgut of the susceptible and Cry1Ac resistant larvae determined by real-time RT-PCR analysis.

<p>Error bars indicate standard errors of the means from analysis of 3 individuals.</p

Response of F₂ larvae from four families of single-pair crosses to Cry1Ac selection.

1<p>Survival Rate (%) was corrected using the Abbott’s formula <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0035991#pone.0035991-Abbott1" target="_blank">[63]</a> with the control survival rates which were ≥95% in the bioassays.</p>2<p>Predicted survival rate 25% to selection with Cry1Ac was calculated based on inheritance of the recessive monogenic Cry1Ac-resistance trait.</p>3<p>Statistical significance was tested by <i>chi</i>-square test.</p

The 200 kDa Cry1Ac-binding cadherin was detected in midgut BBMV proteins from both susceptible and resistant <i>T. ni</i> by toxin overlay binding analysis.

<p>The 200 kDa Cry1Ac-binding cadherin was detected in midgut BBMV proteins from both susceptible and resistant <i>T. ni</i> by toxin overlay binding analysis.</p