Skuteczność oznaczania parametrów morfologii w diagnostyce ciąży ektopowej jajowodowej

Objective: Ectopic pregnancy (EP) is the major cause of maternal morbidity and is responsible for maternal mortality in the first trimester. In order to reduce undesirable results, it is necessary to find rapid and accurate, non-surgical diagnostic tests for EP. The goal of the study was to investigate the differences in complete blood count parameters between tubal EPs and healthy pregnancies in be used in the diagnosis of ectopic pregnancy. Study design: White blood cell (WBC), neutrophil, monocyte, lymphocyte, platelet (PLT) counts, mean PLT volume (MPV) and PLT distribution width (PDW) levels in the complete blood count samples have been obtained from subjects with diagnosed tubal EP (n=78; study group) and women with healthy intrauterine gestations (n=79; control group). Statistical comparisons between groups were performed using the t test. Results: PDW levels were found to be significantly higher in the control group than EP (pCel: Ciąża ektopowa (EP) jest główną przyczyną śmiertelności matek w pierwszym trymestrze ciąży. Aby zmniejszyć niekorzystne wyniki tej choroby, konieczne jest znalezienie szybkiego i właściwego testu nieoperacyjnego służącego do rozpoznania EP. Celem badania była ocena różnic w wynikach morfologii pomiędzy pacjentkami z ciążą ektopową a zdrowymi ciężarnymi. Materiał i metoda: W próbkach pełnej krwi zbadano następujące parametry: leukocyty (WBC), neutrofile, monocyty, limfocyty, płytki krwi (PLT), średnią objętość płytki krwi (MPV), szerokość rozdziału płytek (PDW) u pacjentek z rozpoznaną ciążą jajowodową (n=78, grupa badana) oraz u zdrowych ciężarnych (n=79, grupa kontrolna). Analiza statystyczna porównująca obie grupy została wykonana przy pomocy testu t. Wyniki: PDW był istotnie wyższy w grupie kontrolnej niż w grupie EP (

Vitamin D deficiency in pregnancy may affect fetal thymus development

Objectives: The aim of our study was to evaluate the association of vitamin D deficiency (VDD) during pregnancy with thymus size in full-term fetuses. Material and methods: In this prospective study, we evaluated mid-pregnancy serum 25-hydroxyvitamin D3 (25(OH)D3) concentrations. The fetal thymus size was measured by ultrasound in the third trimester. Neonatal 25(OH)D3 levels were evaluated by umbilical cord blood sampling. Correlation of maternal and neonatal vitamin D levels and association between thymus size and both, maternal and neonatal vitamin D concentrations were investigated. Results: Serum 25(OH) D3 concentrations were within the normal range in 48 (29.8%) mothers and 10 (13.1%) new­borns. A strong correlation between mid-pregnancy maternal and neonatal 25(OH)D3 concentration (r = 0.8, p < 0.001) was found. A significant linear correlation was observed between both, maternal and neonatal 25(OH)D3 concentrations and thymus perimeter length (r = 0.45, p = 0.04 and r = 0.43, p < 0.01, respectively). Both, maternal and fetal VDDs were associated with decreased thymus perimeter (p = 0.04, p = 0.03). Conclusions: Vitamin D deficiency during pregnancy may be associated with smaller fetal thymus. Our data suggest that VDD in pregnancy may lead to systemic inflammatory response in the fetus

Comparison of long-term kidney functions after radical nephrectomy and simple nephrectomy

Objective To determine if there is a difference in proceeding to CKD between patients who had undergone radical nephrectomy (RN) and simple nephrectomy (SN) for different indications by comparing the short- and long-term renal function. Materials and Methods We retrospectively analyzed the records of all patients who underwent nephrectomy (either for malign or benign indications) in our clinic between January 2007 and September 2017. The patients were divided into 2 groups according the type of surgery: 1) Radical nephrectomy Group, 2) Simple Nephrectomy Group. Renal function was evaluated with Glomerular Filtration Rate (GFR) calculated using the MDRD formula. Results A total of 276 patients were included in the study. There were 202 patients in RN Group and 74 patients in SN Group. The mean age of the patients in RN Group and SN Group were age 59,2 ± 11,5 and 49,9 ± 15,1 years, respectively (p = 0.001). GFR levels of patients in RN Group versus SN Group were as follows: Preoperative period: 84.9 vs. 81 mL/min/1.73 m2; postoperative 1st day: 60.5 vs. 84.4 mL/min/1.73 m2, postoperative 1st month 58.9 vs. 76 mL/min/1.73 m2, postoperative 1st year: 59.5 vs. 74.1 mL/min/1.73 m2; at last control 60.3 and 76.1 mL/min/1.73 m2. While preoperative GFR was found to be similar in two groups (p = 0.26), postoperative GFR values were found to be significantly lower in Group RN (p < 0.001). In comparison of the decrease in GFR in two groups at last follow-up, significantly higher decrease was observed in RN Group, 29% vs. 6%, (p < 0.05). Conclusion The decrease in GFR exists more common and intensive after RN compared to SN. In long-term, compensation mechanisms that develop after sudden nephron loss like radical nephrectomy deteriorates kidney function more than gradual nephron loss as in benign etiologies which indicates simple nephrectomy

<em>Chlamydia trachomatis</em>: A Tiny Being beyond the Nature

Chlamydia trachomatis is the most common cause of sexually transmitted genital infections. Females are at high risk of cervix infections, and a significant proportion may also have urethral infections. Pelvic inflammatory disease (PID) can develop as a result of C. trachomatis ascending to the upper reproductive tract. C. trachomatis is an obligate intracellular bacterium that infects the genital tract and may cause chronic inflammation, damage to epithelial tissues, and pelvic inflammation. It has also been clinically associated with cervical atypia and metaplasia. C. trachomatis is the most prevalent sexually transmitted pathogen, and it can cause infertility if left undetected and untreated. Infertile women may be more susceptible to chlamydial infections due to their longer periods of active sexual life. Several diagnostic techniques are available to diagnose chlamydia, including DNA amplification testing (NAAT), culture, antigen detection, and genetic probes; microscopy is not useful for this purpose Chlamydia is treated with empiric therapy, which includes tetracyclines, macrolides, and some fluoroquinolones

Mıcrochıp Based Bacterıa Detectıon Usıng Magnetıc Nanopartıcles And Quantum Dots

Bu çalışmada, bakteri tayini için immunomanyetik ayırma ve floresans etiketleme kullanılarak mikroçip tabanlı bir biyosensör geliştirilmiştir. Çalışma kapsamında öncelikle manyetik nanopartiküllerin ve kuantum noktalarının sentezi ve yüzey modifikasyonları gerçekleştirilmiştir. İmmunomanyetik ayırma ile zenginleştirilmiş bakterilerin floresans etiketleme, yıkama ve sinyal ölçümleri birbirine kapiler vana ile bağlanmış dört odacıklı mikroçip kullanılarak gerçekleştirilmiştir. Mikroçipteki son odacık okuma bölgesi olarak kullanılmıştır. MNP-E.coli-KN konjugatının floresans sinyalleri ışık yayan diyot lambası, fiber optik kablo ve el tipi floresans spektrometresi ile oluşturulan taşınabilir bir düzenekle ölçülmüştür. Yöntemin determinasyon katsayısı ve teşhis sınırı sırasıyla 0,994 ve 3 kob/mL dir. Analiz süresi yaklaşık 45 dakika olarak bulunmuştur. Yöntemin seçiciliği Enterobacter aerogenes, Salmonella enteritidis, Staphylococcus aureus ile test edilmiş ve bu bakterilerin E. coli tayinine girişim etkisinin olmadığı gözlenmiştir. Geliştirilen yöntem, çeşme suyu ve göl suyu numunelerine başarıyla uygulanmıştır; yöntem sahada bakteri tayinine uygunluğu, seçiciliği, geniş dinamik aralığı ve yüksek duyarlılığı ile literatürdeki mevcut biyosensörlere üstünlük sağlamaktadır.In this study, a microchip based biosensor was developed using immunomagnetic separation and fluorescence labelling. First of all, synthesis and surface modifications of magnetic nanoparticles and quantum dots were carried out. After the preconcentration by immunomagnetic separation, fluorescence labelling, washing and signal measurements were performed using a microchip that consists of four microchamber connected to each other with a capillary valve. Last chamber of the microchip was used for signal measurements. Fluorescence signal of MNP-E.coli-QD conjugate was measured using portable equipment consist of light emitting diode lamp, fiber optic probe and hand-held fluorescence spectrometer. The determination coefficient and the limit of detection was 0,994 and 3 cfu/mL, respectively. The analysis time was found approximately 45 minutes. Selectivity of method was tested with Enterobacter aerogenes, Salmonella enteritidis, Staphylococcus aureus and it is observed that these bacteria have no interference effect on E.coli determination. The developed method was successfully applied to tap and lake water samples; the method is suitable for on-site bacteria detection and it is superior to biosensors available in the literature with selectivity, large dynamic range and high sensitivity

Doğum şeklinin seksüel fonksiyon üzerine etkisi var mıdır?

Objective: Sexual dysfunction in women is defined as inability to reach or enjoy orgasm. Some studies have demonstrated that sexual health may be altered by delivery method. This study aimed to investigate whether delivery method affects sexual function during postpartum period in a Turkish female population. Methods: in this prospective study we aimed to compare sexual functions at postpartum 6 to 24 months in primipara patients who gave birth by vaginal delivery (NVD) (n=40) or cesarean section (CS) (n=40) at Şifa Hospital between 2012 and 2014. We used Female Sexual Function Index (FSFI) form as the sexual dysfunction assessment method. the groups were matched with regard to demographic characteristics, medical history, obstetric details, and body mass index values.Results: Apart from body mass index (BMI), the study groups showed no significant differences with regard to age, educational status, frequency of sexual activity, the number of marital years, occupational status, and socioeconomic level. Both groups did not show any significant differences with respect to sexual desire, sexual stimulation, lubrication, dyspareunia, orgasm, and sexual achievement. Conclusion: Our study have demonstrated that vaginal delivery does not adversely affect postnatal sexual function in women.Amaç: Kadınlarda cinsel işlev bozukluğu, orgazma ulaşamama ya da orgazmdan zevk alamama olarak tanımlanmaktadır. Bazı çalışmalarda, cinsel sağlığın doğum yöntemiyle değişebildiği gösterilmiştir. Bu çalışmada, Türkiye'de, kadın popülasyonunda doğum yönteminin postpartum dönemde cinsel fonksiyonu etkileyip etkilemediğini araştırmayı amaçlamaktadır. Yöntemler: Bu prospektif çalışmada, 2012-2014 yılları arasında Şifa Hastanesinde (40) Normal vaginal doğum (NVD) ve (40) sezaryen (CS) ameliyatı olan primipar hastaların doğum sonrası 6.-24. aylarda sexüel fonksiyonlarını karşılaştırmayı amaçladık. Cinsel işlev bozukluğunu değerlendirme yöntemi olarak Kadın Cinsel İşlev İndeksi (FSFI) formunu kullandık. Gruplar demografik özellikler, tıbbi özgeçmiş ve obstetrik detaylar ve vücut kitle indeksi değerleri açısından eşleştirildi.Bulgular: Çalışma grupları arasında vücut kitle indeksi (VKİ) dışında yaş, eğitim düzeyi, sexüel aktivite sıklığı, evlilik yılı, iş durumu ve sosyoekonomik düzey açısından istatistiksel olarak farklılık göstermediler. Her iki grup arasında yapılan karşılaştırmada, istek, uyarılma, lubrikasyon, ağrı, orgazm, cinsel başarı yönünden anlamlı bir fark saptanmamıştır.Sonuç: Çalışmamızda, vajinal doğumun postnatal cinsel işlevleri olumsuz şekilde etkilemediği gösterilmektedir

Anterior sacral meningocele masquerading as an ovarian cyst: a rare clinical presentation associated with Marfan syndrome

Anterior sacral meningocele is a very rare clinical entity characterized by herniation of a meningeal sac through a sacrococcygeal defect. We report a case of a 20-year old female with Marfan syndrome who presented with abdominal distention that was misdiagnosed as an ovarian cyst on pelvic ultrasound. Pelvic magnetic resonance (MR) imaging showed large, well-defined multiloculated intrasacral and presacral cysts communicating via two separate broad necks and extending through defects in anterior aspect of sacral vertebrae. This case emphasizes that anterior sacral meningocele should be considered in the differential diagnosis of cases with pelvic cysts particularly in patients with underlying connective tissue disorders. Because severe neurologic complications or even death may occur without proper preoperative planning in such cases, MR imaging should always be performed for evaluation and characterization of pelvis cystic lesions