PROSPECTS OF STUDYING OF VOLATILE OILS OF TANACETUM PARTHENIUM (L.) SCHULTZ BIP. FOR ISSUES IN CHEMOSYSTEMATICS OF TANACETUM GENUS

The search for new sources of biologically active substances is an actual issue of pharmaceutical science. Feverfew is a prospective member of the Tanacetum genus. Recently an interest to this plant has grown. Sesquiterpene lactones and phenolic compounds are known to be cause its anti-inflammatory and antimigraine activity. The study of volatile compounds and chemosystematics of the feverfew in this group of BAS has not been conducted before. The aim of the research was to obtain the volatile oil from the feverfew herb, studying of the component composition, an identification of compounds, that can be used for chemosystematics of the genus and establishing of the pharmacological activity according to the chemical content of volatile oil. Materials and methods. The object of research was the samples of the feverfew herb grown and collected in the period of its mass flowering. The volatile oil obtained by steam distillation from the feverfew herb was studied using gas chromatography–mass spectrometry. Results. The comparative characteristic of the representatives of the genus Tanacetum according to chemosystematic features was carried out. As a result of the research of the volatile oil 44 compounds were found, from which 40 were identified. According to the research, the raw material contained 34.71 % of camphor, this is a typical feature of all species of the genus Tanacetum. Fenchyl acetate was identified in a high enough content – 7,44 % in the domestic raw material, but this compound was not identified in the foreign samples. Chrysanthenyl acetate is accumulated in representatives of the genus Tanacetum in the form of a cis or trans isomer. In our study, the content of trans-chrysanthene acetate was 23.30 %. In the domestic raw materials, monoterpenes – α- and β- thujone – were not identified, which are associated with the toxicity of the raw material. Conclusions. In the volatile oil 44 compounds were found, from which 40 were identified. Monoterpenes prevailed among terpenes, namely camphor, trans-chrysanthenyl acetate and fenchyl acetate, which are typical and can be used for chemosystematic issues of the genus Tanacetum. The identified compounds require anti-inflammatory, antiseptic, antispasmodic, analgesic activities of the volatile oil, therefore creation of the medicinal herbal remedy based on the volatile oil of the feverfew herb is prospective

Дослідження фенольного профілю, aнтирадикальної та протизапальної активності густого водно-спиртового екстракту трави маруни дівочої (Tanacetum parthenium L.)

The aim – to study the phenolic complex of a thick hydroalcoholic extract of the feverfew (Tanacetum parthenium (L.) herb (FTHAE), its antiradical activity and anti-inflammatory properties in a model of carrageenan and histamine oedema. Materials and methods. The studied extract was obtained from the Tanacetum parthenium herb, collected in Sumy and Poltava regions of Ukraine during the period of mass flowering (June-August): degree of grinding of raw materials 2.0-3.0 mm, extraction temperature - 25 °C, extractant – 70 % ethanol, raw material/extractant ratio – 1:12, infusion time – 12 hours, multiplicity of extractions – 3. HPLC and spectrophotometric methods were used to determine the composition and amount of phenolic compounds of FTHAE. HPLC analysis was performed using a “Waters e2695 Alliance system” (Waters, Milford, MA, USA) with a photodiode array detector “Waters 2998” according to the HPLC–PDA method for phenolic compounds. The scavenging of ABTSA radical cation evaluated the radical scavenging activity. In addition, the anti-inflammatory properties of FTHAE were studied on carrageenan and histamine paw oedema in rats. Anti-inflammatory activity (AIA) was evaluated as the ability to reduce oedema compared to the control pathology. FTHAE was used at a dose of 50 mg/kg. The results. The content of the sum of hydroxycinnamic acids in the obtained extract was determined by spectrophotometry, which was 13.92±0.02 % and the content of the sum of flavonoids – 5.16±0.03 %. The content of 12 compounds with a total amount of 72432.09µg/g was identified and determined by HPLC. The dominant compounds were hydroxycinnamic acids, namely 3,4-dicaffeoylquinic, 4,5-dicaffeoylquinic and сhlorogenic acids. The antiradical activity of the extract was 620.19±4.53µmol/g. On the model of carrageenan oedema, the maximum effect of oedema suppression was 71.0-73.2 %. In the model of histamine oedema, the anti-inflammatory effect of the extract was 57.8; 51.8; and 49.1 % for 30 minutes, 1 and 1.5 hours of oedema, respectively. The severity of the anti-inflammatory activity of the extract during the first hour is not inferior to the diclofenac sodium, quercetin and loratadine. Conclusions. Due to the HPLC method, 12 compounds were determined to cause antiradical activity, among which chlorogenic acid and rutin were identified. The studied extract has a pronounced anti-inflammatory effect, which is due to the antiradical properties of the extract and its inhibitory effect on inflammatory mediatorsМета – вивчити фенольний комплекс густого водно-спиртового екстракту пижми дівочої (Tanacetum parthenium (L.) herb (ПГВСЕ), його антирадикальну активність та протизапальні властивості на моделі карагенінового та гістамінового набряків. Матеріали та методи. Досліджуваний екстракт отримано з трави маруни дівочої, зібраної в Сумській та Полтавській областях України в період масового цвітіння (червень-серпень): ступінь подрібнення сировини 2,0-3,0 мм, температура екстракції – 25 °С, екстрагент. – спирт етиловий 70 %, співвідношення сировина/екстрагент – 1:12, час настоювання – 12 годин, кратність екстракції – 3 рази. Для визначення складу та кількості фенольних сполук ПГВСЕ використовували ВЕРХ та спектрофотометричні методи. ВЕРХ-аналіз проводили за допомогою системи «Waters e2695 Alliance» (Waters, Milford, MA, USA) з фотодіодним детектором «Waters 2998» за методом HPLC–PDA для фенольних сполук. Активність поглинання радикалів оцінювали за допомогою поглинання катіон-радикала АБТСК (2,2'-азінобіс-(3-етилбензотіазолін-6-сульфонова кислота). Протизапальні властивості ПГВСЕ вивчали на карагеніновому та гістаміновому набряках лап у щурів. Протизапальну активність (ПЗА) оцінювали як здатність зменшувати набряк порівняно з такою в групі контрольної патології. ПГВСЕ використовували в дозі 50 мг/кг. Результати. В отриманому екстракті спектрофотометрично визначено вміст суми гідроксикоричних кислот, яка становила 13,92±0,02 %, а вміст суми флавоноїдів – 5,16±0,03 %. Вміст 12 сполук загальною кількістю 72432,09 мкг/г ідентифіковано та визначено методом ВЕРХ. Домінуючими сполуками були гідроксикоричні кислоти, а саме 3,4-дикофеоїлхінна, 4,5-дикафеоілхінна та хлорогенова кислоти. Антирадикальна активність екстракту становила 620,19±4,53 мкмоль/г. На моделі карагенінового набряку максимальний ефект пригнічення набряку становив 71,0-73,2 %. На моделі гістамінового набряку протизапальний ефект екстракту становив 57,8; 51,8; і 49,1 % на 30 хв, 1 і 1,5 год набряку відповідно. За вираженістю протизапальної активності екстракт протягом першої години не поступається диклофенаку натрію, кверцетину та лоратадину. Висновки. Методом ВЕРХ визначено 12 сполук, що виявляють антирадикальну активність, серед яких виявлено хлорогенову кислоту та рутин. Досліджуваний екстракт має виражену протизапальну дію, що зумовлено антирадикальними властивостями екстракту та його інгібуючим впливом на медіатори запаленн

Phenolic Composition and Antioxidant Activity of <i>Tanacetum parthenium</i> Cultivated in Different Regions of Ukraine: Insights into the Flavonoids and Hydroxycinnamic Acids Profile

Tanacetum parthenium, also known as feverfew, is rich in bioactive compounds, namely sesquiterpene lactones, flavonoids, and volatile oils. Sesquiterpene lactones possess anti-migraine activity, while phenolic compounds possess anti-inflammatory and antioxidant action. Phytochemical composition determines the pharmacological activity and so profiling is essential in quality assessment. The study aimed to evaluate cultivated feverfew plants’ phenolic profiles and antioxidant activity. Eleven phenolic compounds were identified in the samples of feverfew in Ukraine. Hydroxycinnamic acids predominate in the quantitative content of all the samples, namely chlorogenic acid, 3,5-dicaffeoylquinic acid, 3,4-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid. The total content of flavonoids ranged from 0.8 to 2.6%; the content of hydroxycinnamic acids varied from 3.3 to 6.5%. The obtained data testify to the prospects of using Ukrainian feverfew as a raw material with a significant content of phenolic substances to develop new herbal medicines