An Extreme Case of a Misaligned Highly Flattened Wind in the Wolf-Rayet Binary CX Cephei

CX Cep (WR 151) is the WR+O binary (WN5+O5V) with the second shortest period known in our Galaxy. To examine the circumstellar matter distribution and to better constraint the orbital parameters and mass-loss rate of the WR star, we obtained broadband and multi-band (i.e. UBVRI) linear polarization observations of the system. Our analysis of the phase-locked polarimetric modulation confirms the high orbital inclination of the system (i.e. $i=65^o$). Using the orbital solution of Lewis et al. (1993) we obtain masses of $33.9 M_{\odot}$ and $23.9 M_{\odot}$ for the O and WR stars respectively, which agree with their spectral types. A simple polarimetric model accounting for finite stellar size effects allowed us to derive a mass-loss rate for the WR star of $0.3-0.5\times10^{-5} M_{\odot}/yr$. This result was remarkably independent of the model's input parameters and favors an earlier spectral type for the WR component (i.e. WN4). Finally, using our multi-band observations, we fitted and subtracted from our data the interstellar polarization. The resulting constant intrinsic polarization of $3-4$ is misaligned in relation to the orbital plane (i.e. $\theta_{CIP}=26^o$ vs. $\Omega=75^o$) and is the highest intrinsic polarization ever observed for a WR star. This misalignment points towards a rotational (or magnetic) origin for the asymmetry and contradicts the most recent evolutionary models for massive stars (Meynet & Maeder 2003) which predict spherically symmetric winds during the WR phase (i.e. $CIP=0$).Comment: 26 pages, 4 figures. Astrophysical Journal (submited

P73 regulates cisplatin-induced apoptosis in ovarian cancer cells via a calcium/calpain-dependent mechanism

P73 is important in drug-induced apoptosis in some cancer cells, yet its role in the regulation of chemosensitivity in ovarian cancer (OVCA) is poorly understood. Furthermore, if and how the deregulation of p73-mediated apoptosis confers resistance to cisplatin (CDDP) treatment is unclear. Here we demonstrate that TAp73α over-expression enhanced CDDP-induced PARP cleavage and apoptosis in both chemosensitive (OV2008 and A2780s) and their resistant counterparts (C13* and A2780cp) and another chemoresistant OVCA cells (Hey); in contrast, the effect of ΔNp73α over-expression was variable. P73α downregulation attenuated CDDP-induced PUMA and NOXA upregulation and apoptosis in OV2008 cells. CDDP decreased p73α steady-state protein levels in OV2008, but not in C13*, although the mRNA expression was identical. CDDP-induced p73α downregulation was mediated by a calpain-dependent pathway. CDDP induced calpain activation and enhanced its cytoplasmic interaction and co-localization with p73α in OV2008, but not C13* cells. CDDP increased the intracellular calcium concentration ([Ca2+]i) in OV2008 but not C13* whereas cyclopiazonic acid (CPA), a Ca2+-ATPase inhibitor, caused this response and calpain activation, p73α processing and apoptosis in both cell types. CDDP-induced [Ca2+]i increase in OV2008 cells was not effected by the elimination of extracellular Ca2+, but this was attenuated by the depletion of internal Ca2+ store, indicating that mobilization of intracellular Ca2+] stores was potentially involved. These findings demonstrate that p73α and its regulation by the Ca2+-mediated calpain pathway are involved in CDDP-induced apoptosis in OVCA cells and that dysregulation of Ca2+/calpain/p73 signaling may in part be the pathophysiology of CDDP resistance. Understanding the cellular and molecular mechanisms of chemoresistance will direct the development of effective strategies for the treatment of chemoresistant OVCA

Estimation of biological recultivation results of the Korkino coal pit upper terraces

Приведены результаты обследования 10-летних посадок деревьев и кустарников на верхних уступах Коркинского угольного разреза. Сравнительный анализ строения корневых систем и ежегодного прироста в толщину показал перспективность насаждений тополя бальзамического при биологической рекультивации разреза.The paper presents the results of the investigation of trees and bushes planted 10 years ago at the upper terraces of the Korkino coal pit. Comparative analysis of root system structure and annual thickness increment shows the perspectiveness of the balsam poplar (Populus balsamifera L.) plantings for biological recultivation of the pit