Overview of selective hydrogen peroxide quantitation.

<p>A.) Chemical reaction between hydrogen peroxide and cerium (IV) sulfate. B.) Chemical reaction between peracetic acid and cerium (IV) sulfate. C.) Multiple wavelength cerium (IV) sulfate standard curve (n = 4). Error bars represent standard deviation and are typically smaller than the plotted marker.</p

Comparison of hydrogen peroxide quantitation.

<p>The amount of hydrogen peroxide in various dilutions from a stock peracetic acid/hydrogen peroxide solution were determined via titration or the microtiter plate based method (n = 3, error bars represent standard deviation).</p

A High-Throughput Microtiter Plate Based Method for the Determination of Peracetic Acid and Hydrogen Peroxide

<div><p>Peracetic acid is gaining usage in numerous industries who have found a myriad of uses for its antimicrobial activity. However, rapid high throughput quantitation methods for peracetic acid and hydrogen peroxide are lacking. Herein, we describe the development of a high-throughput microtiter plate based assay based upon the well known and trusted titration chemical reactions. The adaptation of these titration chemistries to rapid plate based absorbance methods for the sequential determination of hydrogen peroxide specifically and the total amount of peroxides present in solution are described. The results of these methods were compared to those of a standard titration and found to be in good agreement. Additionally, the utility of the developed method is demonstrated through the generation of degradation curves of both peracetic acid and hydrogen peroxide in a mixed solution.</p></div

Effect of sulfuric acid concentration on peroxide – potassium iodide reaction.

<p>A.) Various concentrations of sulfuric acid were used to react (A) 15 mM total peroxides with 250 mM potassium iodide or (B) only 250 mM potassium iodide (n = 3). Error bars represent standard deviation.</p

Comparison of total peroxide quantitation.

<p>The amount of total peroxide in various dilutions from a stock peracetic acid/H₂O₂ solution were determined via titration or the microtiter plate based method (n = 3, error bars represent standard deviation).</p

Overview of general peroxide quantitation.

<p>A.) Chemical reaction between hydrogen peroxide and potassium iodide. B.) Chemical reaction between peracetic acid and potassium iodide. C.) Multiple wavelength iodine standard curve (n = 4). Error bars represent standard deviation and are typically smaller than the plotted marker.</p

Selective Tumor Targeting of Desacetyl Vinblastine Hydrazide and Tubulysin B via Conjugation to a Cholecystokinin 2 Receptor (CCK2R) Ligand

As the delivery of selectively targeted cytotoxic agents via antibodies or small molecule ligands to malignancies has begun to show promise in the clinic, the need to identify and validate additional cellular targets for specific therapeutic delivery is critical. Although a multitude of cancers have been targeted using the folate receptor, PSMA, bombesin receptor, somatostatin receptor, LHRH, and α_vβ₃, there is a notable lack of specific small molecule ligand/receptor pairs to cellular targets found within cancers of the GI tract. Because of the selective GI tract expression of the cholecystokinin 2 receptor (CCK2R), we undertook the creation of conjugates that would deliver microtubule-disrupting drugs to malignancies through the specific targeting of CCK2R via a high affinity small molecule ligand. The cytotoxic activity of these conjugates were shown to be receptor mediated in vitro and in vivo with xenograft mouse models exhibiting delayed growth or regression of tumors that expressed CCK2R. Overall, this work demonstrates that ligands to CCK2R can be used to create selectively targeted therapeutic conjugates