Is the Colonisation of Staphylococcus aureus in Pets Associated with Their Close Contact with Owners?

In human beings and animals, staphylococci constitute part of the normal microbial population. Staphylococcus aureus could be classified as an opportunistic pathogen because the bacteria are noted in clinically healthy individuals, but when the immune system becomes compromised, they can also cause a wide range of infections. The objective of this study was to test the hypothesis that cats who are in close contact with their owners are at the greatest risk of being colonised with S. aureus. Two groups of cats were investigated: single, pet (domestic) cats that do not have outdoor access; and a local population of feral cats living in urban areas. The prevalence of S. aureus in domestic cats was 19.17%, while it's prevalence in the feral cat population was only 8.3%; which was statistically significant. Analysis of antibiotic resistance, at the genotypic as well as phenotypic level, showed that S. aureus isolates from pet cats were more likely to harbour antibiotic resistant determinants. The prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in households was 10.21%, while in feral cats it was only 1.4%. In conclusion, this study has revealed a correlation between close contact with humans and a higher risk of the cats being colonised with S. aureus and harbouring the antibiotic resistant determinants

Virulence Genes as Markers for <i>Pseudomonas aeruginosa</i> Biofilm Formation in Dogs and Cats

Pseudomonas aeruginosa is an ubiquitous bacterium and opportunistic pathogen that plays an important role in nosocomial infections. The presence of virulence factors and the biofilm-forming ability of this species contributes to a high risk of treatment complications. In this study, we examined the biofilm-forming ability and the prevalence of five virulence factor genes (pslA, pelA, ppyR, fliC, and nan1) in 271 P. aeruginosa isolates (212 from dogs and 59 from cats). Biofilm-forming ability was detected in 90.6% of isolates in dogs and 86.4% of isolates in cats. In P. aeruginosa isolates from both species, the most prevalent virulence factor gene was ppyR (97.2% in dogs and 98.3% in cats), followed by pslA (60.8% and 57.6%), fliC (60.4% and 69.5%), nan1 (45.3% and 44.1%), and pelA (40.1% and 33.9%, respectively). In dogs, a significantly higher proportion of biofilm-forming P. aeruginosa strains possessed the fliC gene compared to non-biofilm-forming strains (p = 0.015). In cats, a significantly lower proportion of biofilm-forming strains had the nan1 gene compared to non-biofilm-forming strains (p = 0.017). In conclusion, the presence of fliC gene and the absence of nan1 gene could be indicators of biofilm-forming ability of P. aeruginosa

Prevalence of <i>S</i>. <i>aureus</i> in pet cats and feral cats, including combinations of sampling places.

<p>Sampling places: nares (N); conjunctival sacs (O); anus (P); skin (S). For each combination of sampling places a confidence interval was marked which was calculated using the bootstrap method.</p

Bacterial Diversity in Feline Conjunctiva Based on 16S rRNA Gene Sequence Analysis: A Pilot Study

Changes in the microbial populations in the conjunctival sacs of animals have traditionally been evaluated using conventional microbiology techniques. The goal of this study was to examine the suitability of a methodology which may reveal a previously unknown microbiome inhabiting feline conjunctival membranes. In the present study, we determined the microbial diversity in feline conjunctivas based on 16S rRNA gene sequence analysis. Five taxa not described earlier in veterinary ophthalmology (i.e., Staphylococcus caprae, Staphylococcus succinus, Propionibacterium acnes, Psychrobacter faecalis, and Bacillus subtilis) were identified in feline conjunctivas with a high similarity (99-100%). The study demonstrates that the feline conjunctival sacs are inhabited by much more rich and diverse microbial communities than previously thought using culture-based methods. From the clinical perspective, this could suggest that other laboratory procedures (e.g., extended incubation time in the case of Actinobacteria, formerly order Actinomycetales) or a new tool like culture-independent approaches (next-generation DNA sequencing) should be taken into account

Diversity of <i>spa</i> types and antibiotic resistance patterns in pet cat and feral cat groups.

<p>Diversity of <i>spa</i> types and antibiotic resistance patterns in pet cat and feral cat groups.</p

Percentage of genetic determinants of the antibiotic resistance among isolates of <i>S</i>. <i>aureus</i>.

<p>Percentage of genetic determinants of the antibiotic resistance among isolates of <i>S</i>. <i>aureus</i>.</p

Prevalence of MRSA in pet cats and feral cats, including combinations of sampling places.

<p>Sampling places: nares (N); conjunctival sacs (O); anus (P); skin (S). For each combination of sampling places a confidence interval was marked which was calculated using the bootstrap method.</p

Percentage of antibiotic resistance according MIC test in all isolated <i>S</i>. <i>aureus</i> strains.

<p>Percentage of antibiotic resistance according MIC test in all isolated <i>S</i>. <i>aureus</i> strains.</p