<i>TP53</i> genotype frequency in patients and controls.

<p>The values in parentheses are the number of individuals the frequency is based on.</p><p>There were no significant differences between patients and controls.</p

Kaplan-Meier analysis of survival in a cohort of IPF patients.

<p>(A) Carriers of the <i>TP53</i> rs12951053 C or rs12602273 G alleles had significantly worse 4-year survival rate (p = 0.006). (B) There was no significant difference in survival between carriers and non-carriers of the <i>CDKN1A</i> rs2395655 G allele (p = 0.2).</p

Linkage disequilibrium plot.

<p>Showing pairwise r² for SNPs in <i>TP53</i> (A) and <i>CDKN1A</i> (B).</p

<i>CDKN1A</i> mRNA expression in healthy controls.

<p><i>CDKN1A</i> mRNA levels were significantly different between rs733590 genotypes, p = 0.03 (TT+CT vs. CC). A similar trend was observed for rs2395655 genotypes (p = 0.06, AA+AG vs. GG).</p

Genotype and lung function decline.

<p>Number of IPF patients with non-rapid or rapid decline in %predicted vital capacity (>10% in one year) or %predicted diffusion capacity (>15% in one year).</p><p>*Fisher's exact test with carriers of p21 rs2395655G vs. non-carriers resulted in p = 0.04.</p

<i>CDKN1A</i> genotype and carriership frequency in patients and controls.

<p>The values in parentheses are the number of individuals. P values are based on the number of individuals with and without the specified genotype and are calculated using a Pearson's goodness-of-fit chi-square test. Odds ratio (OR) is shown with the 95% confidence interval in brackets.</p><p>*After correction for multiple testing rs733590 and rs2395655 remained significant.</p

Correlation between FISH-TL and MMqPCR leukocyte TL (blood T/S) in FIP-TERT fibrotic areas.

<p>Positive FIP-TERT correlation (Spearman) between alveolar type 2 (AT2) cell FISH-TL in fibrotic areas and MMqPCR blood T/S (n = 9, r² = 0.67, p = 0.007).</p

FISH telomere length in lungs of FIP-TERT and FIP-nonTERT subjects.

<p>(<b>A</b>) Median FISH-TL in alveolar type 2 (AT2) cells in non-fibrotic and fibrotic areas of 9 FIP-TERT subjects. Telomeres are generally short and no significant difference between areas was observed (2-tailed, p = 0.36). (<b>B, C</b>) Median FISH-TL of same subjects as in figure <b>A</b>, showing differences between surrounding and AT2 cells in (<b>B</b>) non-fibrotic and (<b>C</b>) fibrotic areas. (<b>D)</b> Median FISH-TL in alveolar type 2 (AT2) cells in non-fibrotic and fibrotic areas of 10 FIP-nonTERT subjects. Non-fibrotic AT2 FISH-TL is significantly higher compared to FISH-TL in fibrotic areas (2-tailed, p = 0.02). (<b>E, F</b>) Median FISH-TL of same subjects as in figure <b>D</b>, showing differences between surrounding and AT2 cells in (<b>E</b>) non-fibrotic and (<b>F</b>) fibrotic areas. Asterisks indicate significant differences calculated by Wilcoxon matched-pairs signed rank analysis (* = p<0.05, ** = p<0.01).</p

Telomere length in sporadic IPF lungs.

<p>(<b>A</b>) Representative example of telomere length per alveolar type 2 (AT2) cell for one sporadic IPF subject. Each dot represents one cell. Non-fibrotic AT2 FISH-TL is significantly higher compared to FISH-TL in fibrotic areas (p<0.0001). Bars represent medians and p-value is calculated using a Mann-Whitney test. (<b>B, C, D</b>) Median surrounding and AT2 cell FISH-TL in non-fibrotic and fibrotic areas for 16 sporadic IPF subjects. Each line connects, per subject, the FISH-TL of (<b>B</b>) AT2 cells in the non-fibrotic and fibrotic area or median FISH-TL differences between surrounding and AT2 cells in (<b>C</b>) non-fibrotic areas or (<b>D</b>) fibrotic areas. AT2 FISH-TL differences between non-fibrotic and fibrotic areas and between surrounding and AT2 cells were significant (2-tailed p<0.0006 and p<0.0001 respectively), which were indicated by asterisks (*** = p<0.001, **** = p<0.0001).</p

Correlation between FISH-TL and biopsy T/S in IPF subjects.

<p>Correlation (Spearman) between FISH-TL measured in alveolar type 2 (AT2) cells and biopsy T/S measured by MMqPCR (n = 15) in non-fibrotic areas (black dots) and fibrotic areas (open squares). Significant correlations were established between biopsy T/S and AT2 cell FISH-TL in non-fibrotic (r² = 0.53, p = 0.002) and fibrotic (r² = 0.73, p<0.0001) areas.</p