Relative gene expression of inflammatory biomarkers in HC11 cells treated with <i>S</i>. <i>aureus</i>.

<p>Differentiated HC11 cells were treated with <i>S</i>. <i>aureus</i> for 2 hours. Gene expressions of the inflammatory biomarkers <i>Cxcl2</i>, <i>Il6 and Tnfα</i> were determined. Normalized gene expressions are presented as means ± SD; n = 12 pooled from 2 separate experiments. Statistically significant differences compared to vehicle controls *p < 0.05; **p < 0.01, *** p < 0.001.</p

Relative gene expression of inflammatory biomarkers in HC11 cells treated with LPS.

<p>Differentiated HC11 cells were treated with LPS for 24 hours. Gene expressions of the inflammatory biomarkers <i>Cxcl2</i>, <i>Il6 and Tnfα</i> were determined. Normalized gene expressions are presented as means ± SD; n = 6. Statistically significant differences compared to vehicle controls *p < 0.05; **p < 0.01.</p

Regression plots with individual data from 2 hours treatment of HC11 cells with <i>S</i>. <i>aureus</i>.

<p>Data from Figs <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0161346#pone.0161346.g002" target="_blank">2</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0161346#pone.0161346.g003" target="_blank">3</a> were analysed by simple linear regression analysis of dependent variable gene expression of transporters (<i>Bcrp</i>, <i>Mdr1</i>, <i>Mrp1</i>, <i>Oatp1a5</i>, <i>Octn1</i> and <i>Oct1</i>) or <i>Csn2</i> and independent variable gene expression of inflammatory biomarkers (<i>Cxcl2</i>, <i>Il6 and Tnfα</i>). p < 0.001 in all regressions.</p

Relative gene expression of <i>Csn2</i> and transporters in HC11 cells treated with LPS.

<p>Differentiated HC11 cells were treated with LPS for 24 hours. Expressions of the genes encoding β-casein, <i>Csn2</i>, and the transporters <i>Bcrp</i>, <i>Mdr1</i>, <i>Mrp1</i>, <i>Oatp1a5</i>, <i>Octn1</i> and <i>Oct1</i> were determined. Normalized gene expressions are presented as means ± SD; n = 6. Statistically significant differences compared to vehicle controls **p < 0.01.</p

Data_Sheet_1_A multi-country One Health foodborne outbreak simulation exercise: cross-sectoral cooperation, data sharing and communication.PDF

IntroductionThe awareness of scientists and policy makers regarding the requirement for an integrated One Health (OH) approach in responding to zoonoses has increased in recent years. However, there remains an overall inertia in relation to the implementation of practical cross-sector collaborations. Foodborne outbreaks of zoonotic diseases continue to affect the European population despite stringent regulations, evidencing the requirement for better ‘prevent, detect and response’ strategies. Response exercises play an essential role in the improvement of crisis management plans, providing the opportunity to test practical intervention methodologies in a controlled environment.MethodsThe One Health European Joint Programme simulation exercise (OHEJP SimEx) aimed at practicing the OH capacity and interoperability across public health, animal health and food safety sectors in a challenging outbreak scenario. The OHEJP SimEx was delivered through a sequence of scripts covering the different stages of a Salmonella outbreak investigation at a national level, involving both the human food chain and the raw pet feed industry.ResultsA total of 255 participants from 11 European countries (Belgium, Denmark, Estonia, Finland, France, Italy, Norway, Poland, Portugal, Sweden, the Netherlands) took part in national level two-day exercises during 2022. National evaluations identified common recommendations to countries aiming to improve their OH structure to establish formal communication channels between sectors, implement a common data sharing platform, harmonize laboratory procedures, and reinforce inter-laboratory networks within countries. The large proportion of participants (94%) indicated significant interest in pursuing a OH approach and desire to work more closely with other sectors.DiscussionThe OHEJP SimEx outcomes will assist policy makers in implementing a harmonized approach to cross-sector health-related topics, by highlighting the benefits of cooperation, identifying gaps in the current strategies and suggesting actions required to better address foodborne outbreaks. Furthermore, we summarize recommendations for future OH simulation exercises, which are essential to continually test, challenge and improve national OH strategies.</p