Additional file 1: Figure S1. of Influence of weaning methods on the diaphragm after mechanical ventilation in a rat model

Arterial blood gas data in the CMV, PSV and SBT groups. Values are expressed as means ± SD. (JPG 717 kb

4 HNE / oxyblot / alpha-spectrin

Western blot

FF measurements / CSA data

force frequency measurements <div>cross-sectional areas</div

Examples of diaphragmatic leukocyte activity

<p>Diaphragmatic leukocyte activity measured as neutrophils (CD43), ED1+ macrophages (CD68), and ED2+ macrophages (CD163). </p

Additional file 1: of Vitamin D deficiency exacerbates COPD-like characteristics in the lungs of cigarette smoke-exposed mice

The effect of vitamin D deficiency on additional lung function parameters in air- and CS-exposed mice after 6 and 12 weeks. (PDF 96 kb

mRNA relative expression of inflammatory cytokines in ventilated animals compared to controls.

<p>Panel (A) shows changes in diaphragm muscle tissue, panel (B) changes in gastrocnemius muscle tissue. * indicate p<0.05. IL  =  Interleukin TNF = Tumor necrosis factor. RQ relative quantity. Further explanation see text. The horizontal line, box, and whiskers of each box plot represent the median, the interquartile range, and the upper and lower range of the data, respectively. n = 8/per group.</p

mRNA relative expression of angio-neogenetic factors in ventilated animals compared to unventilated controls.

<p>Panel (A) shows changes in diaphragm muscle tissue, panel (B) changes in gastrocnemius muscle tissue. ** indicate p<0.001, *** p<0.0001. Ang = Angiopoietin, RQ relative quantity. Further explanation see text. The horizontal line, box, and whiskers of each box plot represent the median, the interquartile range, and the upper and lower range of the data, respectively. n = 8/per group.</p

p-values and fold-changes of mRNA expression in diaphragm and mixed gastrocnemius tissue of MV compared to control.

*<p>indicates significant change versus control.</p

mRNA relative expression of hypoxia regulated transcription factors in ventilated animals compared to controls.

<p>Panel (A) shows changes in diaphragm muscle tissue, panel (B) changes in gastrocnemius muscle tissue. ** indicate p<0.001, *** p<0.0001. HIF = hypoxia inducible factor, VEGF = vascular endothelial growth The horizontal line, box, and whiskers of each box plot represent the median, the interquartile range, and the upper and lower range of the data, respectively. n = 8/group.</p

Changes in capillary/ fiber ratio in both tissues.

<p>Panel (A) shows an example of the staining in diaphragmatic tissue, Panel (B) depicts the capillary/fiber ratio in both tissues. Red dots in the picture indicate capillaries, while blue ones are DAPI stained nuclei. n = 8/group, control diaphragm n = 7; * p<0.05.</p