Localization of SIV infected cells in LN from SM and PM.

<p>Infected cells, as quantified by ISH for SIV RNA. Histomorphological localization of infected cells (identified by dark blue NBT/BCIP chromogen) in GC versus paracortical microenvironments was accomplished by microscopic analysis of Nuclear Fast Red counterstain. Scoring is based on the average for each group at each time point, divided into quartiles (+; 0–5/mm²) (++; 6–15/mm²); (+++; 16–50/mm²); (++++; >50/mm²) derived from quantitative measurements. Asterisk (*) Indicates one germinal center contained an SIV-infected cell at 2, 6, and 24 wpi in a CD8 depleted SM. Numbers of GC evaluated at each time-point is indicated by n values.</p

Immunohistochemistry for CD4, CD8, and IBA-1 in GC of SM and PM.

*<p>indicate accompanying image in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0057785#pone-0057785-g004" target="_blank">Fig. 4</a>.</p><p>Mean score for DAB staining at each time point post-SIV inoculation. PM have higher CD4 scores at 2, 5, and 21 wpi compared to SM at similar time-points. PM also had slightly increased numbers of IBA-1⁺ cells (macrophages) at 2 wpi compared to SM. CD8 scores are slightly higher for PM at 5 and 21 wpi. Overall number of CD8⁺ cells in GC of both species is extremely low. Asterisks indicate accompanying image in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0057785#pone-0057785-g004" target="_blank">Figure 4A</a>.</p