Toxicological evaluation <i>in silico</i> and <i>in vivo</i> of secondary metabolites of <i>Cissampelos sympodialis</i> in <i>Mus musculus</i> mice following inhalation

<p>The ethanolic extract of the leaves of <i>Cissampelos sympodialis</i> showed great pharmacological potential, with inflammatory and immunomodulatory activities, however, it showed some toxicological effects. Therefore, this study aims to verify the toxicological potential of alkaloids of the genus <i>Cissampelos</i> through <i>in silico</i> methodologies, to develop a method in LC-MS/MS verifying the presence of alkaloids in the infusion and to evaluate the toxicity of the infusion of the leaves of <i>C. sympodialis</i> when inhaled by Swiss mice. Results <i>in silico</i> showed that alkaloid 93 presented high toxicological potential along with the products of its metabolism. LC-MS/MS results showed that the infusion of the leaves of this plant contained the alkaloids warifteine and methylwarifteine. Finally, the <i>in vivo</i> toxicological analysis of the <i>C. sympodialis</i> infusion showed results, both in biochemistry, organ weights and histological analysis, that the infusion of <i>C. sympodialis</i> leaves presents a low toxicity.</p