GROWTH IMPROVEMENT OF TOMATO WITH THE APPLICATION OF BACTERIAL ISOLATES PRODUCING INDOLE ACETIC ACID (IAA) AND PHOSPHATE SOLUBILIZER

Soil bacteria have important roles in biogeochemical cycle for soil fertility and have been manipulated for ecologically-friendly crop production.  The search for beneficial association between microbes and plants for promoting growth and health should be studied for tomato growth improvement. The study aimed to  evaluate 19 microbial isolates which produced indole acetic acid (IAA) affecting growth and development of tomato (Palupi variety), and  molecularly identify the most effective isolates in improving tomato growth based on 16s rDNA sequences. The experiment was conducted in pots using a complete randomized design with three replications. The parameters observed included plant height, plant dry weight, root length, root dry weight, and fruit fresh weight.  The isolates that significantly improved tomato growth were molecularly identified using 16s rRNA sequence. The phenotypic properties such as IAA content and phosphate solubilizing index (PI) of the superior isolates were determined. Results showed that the application of bacterial isolates on tomato significantly increased plant dry weight and fruit yield. From 19 isolates tested, Aj 3.7.1.14 significantly increased plant dry weight, root length, and fruit yield. This isolate produced IAA of about 14.77 ppm and PI of 1.86.  Molecular analysis on Aj 3.7.1.14 demonstrated that the isolate had 89% similarity to Pseudomonas fragi. The identified P. fragi was found to be the most effective isolate for improving tomato growth and fruit yield. Another isolate, Bacillus amyloliquefaciens was found to promote root length, root dry weight, and fruit yield. These isolates are potential to be further investigated for field trial

Research of formation process of calcium-phosphate layer on suffer of polilactyd and composite based on its

In this investigation we researched process of formation of calcium-phosphate layer on surface of polylactide and composite materials. We investigate samples in SBF-solution which imitate biological environment conditions. Analysis shown that there is an active forming of new calcium-phosphate layer on the surface by chemical adsorption

Kemantapan Agregat Tanah pada Lahan Produksi Rendah dan Tinggi di PT Great Giant Pineapple

Penelitian ini bertujuan untuk mengetahui pengaruh kemantapan agregat tanah terhadap hasil produksi. Penelitian ini dilaksanakan dari bulan september sampai pada bulan Juli 2015 di PT Great Giant Pineapple (PT. GGP). Lampung Tengah. Penelitian ini mengunakan metode survei. Untuk pengambilan sampel dilakukan secara langsung di PT. GGP pada lokasi lahan 26B produksi rendah dan produksi tinggi. Lokasi 26B mempunyai luas lahan kurang lebih 8,30 ha dan terbagi menjadi 2 petak yang dipisahkan oleh jalan. Penentuan titik sampel secara diagonal,masing-masing di ambil 3 titik di lahan produksi rendah dan produksi tinggi. Pembuatan minipit dibuat untuk menentukan warna tanah dan pengambilan sampel tanah diseetiap titik, pengambilan contoh tanah diambil disetiap kedalaman dari 0-20, 20-40, 40-60. analisis contoh tanah dilakukan di Laboratorium Ilmu Tanah, Jurusan Agroteknologi, Fakultas Pertanian, Universitas Lampung untuk mengetahui sifat fisik tanah. Untukperlakuan analisis sampel tanah dipisahkan disetiap kedalaman, sehingga diperoleh 1 titik ada 3 sampel tanah pada kedalaman 0-20, 20-40, 40-60 dan pengambilan sampel tanah ada sebanyak 6 titik, 3 titik produksi rendah dan 3 titik di produksi tinggi sehingga jumlah sampel keseluruhan ada 18 sampel tanah. Tanah sebelum dianalisis dilakukan pengeringan udara sela 1-2 hari, hal ini dilakukan untuk mendapatkan kapasitas lapang. Untuk menentukan kelas kemantapan agregat perlu dilakukananalisis dengan menggunakan metode ayakan kering dan basah, dan didapatkan nilai RBD pengayakan kering dan RBD pengayakan basah. Kemudian dilakukan perhitungan indeks kemantapan agregat dan hasil nilai dari indeks kemantapan agergat dimasukan kedalam klasifikasi kemantapan agregat untuk mengetahui hasil sampel tanah masuk dalam kelas kemantapan mantap atau tidak mantap. Hasil penelitian menunjukkan bahwa pada lokasi lahan 26B baik yang produksi rendah maupun produksi tinggi sama-sama mempunyai kemantapan agregat sangat mantap

Genetic Diversity of Jatropha spp. Germplasm Based on Morphological and Molecular Markers

Germplasm of Jatropha spp. with high genetic diversity is needed to develop a new superior variety of Jatropha spp. Morphological and molecular characterizations are important to support development of their superior hybrid varieties. The aims of this study were to identify Jatropha spp. accesion potential for genetic improvement using morphological characters and analyze their genetic diversity using SSR markers. A total of eight genotypes of Jatropha spp. originated from several localities in Indonesia and Thailand was observed. Results showed that accessions of Jatropha spp. were varied in morphological and molecular characters. Based on principle component analysis, characters of stem color, leaf veins, leaf shapes, flower position, total branch number, productive branch number, petiole color, and petal color contributed most to the total diversity. Based on oil seed content, potential accessions identified for further genetic improvement were J. podagrica (34.63%) and J. curcas (29.64%). The results of molecular analysis showed that high allele variation (3–7 alleles) was observed among Jatropha spp. accessions with an average allele number of 4.12 and the average Polymorphism Information Content (PIC) value was 0.57 (0.46–0.77). Three SSR markers showed PIC value >0.5 indicating that these markers were informative for genetic diversity detection of Jatropha spp. The phylogenetic analysis showed that seven accessions of Jatropha spp. could be divided in two groups at similarity coefficent of 0.53. Results of genetic diversity analysis in this study should be useful for proper identification and selection for appropriate parents to assist in breeding of Jatropha spp. in Indonesia